Characterization of the Drosophila ortholog of mouse eIF-3p48/INT-6

Shukichi Miyazaki; Susan Rasmussen; Akira Imatani; Francesca Diella; David T. Sullivan; Robert Callahan

doi:10.1016/S0378-1119(99)00130-4

Characterization of the Drosophila ortholog of mouse eIF-3p48/INT-6

Shukichi Miyazaki, Susan Rasmussen, Akira Imatani, Francesca Diella, David T. Sullivan, Robert Callahan

Research output: Contribution to journal › Article › peer-review

Abstract

The mouse mammary tumor virus (MMTV) has been shown to integrate frequently into INT-6 in MMTV-induced mouse mammary tumors. The INT6 gene has been highly conserved through evolution and has recently been shown to encode the p48 component of the eucaryotic translation initiation factor 3 (eIF-3) complex. We report here the isolation of the Drosophila eIF-3p48/INT-6. The gene comprises three exons within 1.8 kb of genomic DNA located at cytogenetic position 73C2 in the Drosophila genome. The 1.5 kb eIF-3p48/INT-6 RNA species encodes a protein composed of 364 amino-acid residues whose sequence is 71% similar to that of the mouse/human eIF-3/INT-6 amino-acid sequence. eIF-3p48/INT-6 RNA is expressed throughout development in Drosophila and the encoded protein is associated with the microsomal subcellular fraction.

Original language	English
Pages (from-to)	241-247
Number of pages	7
Journal	Gene
Volume	233
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-1119(99)00130-4
Publication status	Published - 1999 Jun 11
Externally published	Yes

Keywords

Eucaryotic translation initiation factor 3
Mammary tumor gene INT6

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/S0378-1119(99)00130-4

Cite this

@article{f11f61254fab456bb74f117379e4bbea,

title = "Characterization of the Drosophila ortholog of mouse eIF-3p48/INT-6",

abstract = "The mouse mammary tumor virus (MMTV) has been shown to integrate frequently into INT-6 in MMTV-induced mouse mammary tumors. The INT6 gene has been highly conserved through evolution and has recently been shown to encode the p48 component of the eucaryotic translation initiation factor 3 (eIF-3) complex. We report here the isolation of the Drosophila eIF-3p48/INT-6. The gene comprises three exons within 1.8 kb of genomic DNA located at cytogenetic position 73C2 in the Drosophila genome. The 1.5 kb eIF-3p48/INT-6 RNA species encodes a protein composed of 364 amino-acid residues whose sequence is 71% similar to that of the mouse/human eIF-3/INT-6 amino-acid sequence. eIF-3p48/INT-6 RNA is expressed throughout development in Drosophila and the encoded protein is associated with the microsomal subcellular fraction.",

keywords = "Eucaryotic translation initiation factor 3, Mammary tumor gene INT6",

author = "Shukichi Miyazaki and Susan Rasmussen and Akira Imatani and Francesca Diella and Sullivan, {David T.} and Robert Callahan",

year = "1999",

month = jun,

day = "11",

doi = "10.1016/S0378-1119(99)00130-4",

language = "English",

volume = "233",

pages = "241--247",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Characterization of the Drosophila ortholog of mouse eIF-3p48/INT-6

AU - Miyazaki, Shukichi

AU - Rasmussen, Susan

AU - Imatani, Akira

AU - Diella, Francesca

AU - Sullivan, David T.

AU - Callahan, Robert

PY - 1999/6/11

Y1 - 1999/6/11

N2 - The mouse mammary tumor virus (MMTV) has been shown to integrate frequently into INT-6 in MMTV-induced mouse mammary tumors. The INT6 gene has been highly conserved through evolution and has recently been shown to encode the p48 component of the eucaryotic translation initiation factor 3 (eIF-3) complex. We report here the isolation of the Drosophila eIF-3p48/INT-6. The gene comprises three exons within 1.8 kb of genomic DNA located at cytogenetic position 73C2 in the Drosophila genome. The 1.5 kb eIF-3p48/INT-6 RNA species encodes a protein composed of 364 amino-acid residues whose sequence is 71% similar to that of the mouse/human eIF-3/INT-6 amino-acid sequence. eIF-3p48/INT-6 RNA is expressed throughout development in Drosophila and the encoded protein is associated with the microsomal subcellular fraction.

AB - The mouse mammary tumor virus (MMTV) has been shown to integrate frequently into INT-6 in MMTV-induced mouse mammary tumors. The INT6 gene has been highly conserved through evolution and has recently been shown to encode the p48 component of the eucaryotic translation initiation factor 3 (eIF-3) complex. We report here the isolation of the Drosophila eIF-3p48/INT-6. The gene comprises three exons within 1.8 kb of genomic DNA located at cytogenetic position 73C2 in the Drosophila genome. The 1.5 kb eIF-3p48/INT-6 RNA species encodes a protein composed of 364 amino-acid residues whose sequence is 71% similar to that of the mouse/human eIF-3/INT-6 amino-acid sequence. eIF-3p48/INT-6 RNA is expressed throughout development in Drosophila and the encoded protein is associated with the microsomal subcellular fraction.

KW - Eucaryotic translation initiation factor 3

KW - Mammary tumor gene INT6

UR - http://www.scopus.com/inward/record.url?scp=0032809197&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032809197&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(99)00130-4

DO - 10.1016/S0378-1119(99)00130-4

M3 - Article

C2 - 10375641

AN - SCOPUS:0032809197

SN - 0378-1119

VL - 233

SP - 241

EP - 247

JO - Gene

JF - Gene

IS - 1-2

ER -

Characterization of the Drosophila ortholog of mouse eIF-3p48/INT-6

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this