Characterization of the Lectin from the Skin Mucus of the Kingklip Genypterus capensis

Michitoshi Toda; Rina Goto-Nance; Koji Muramoto; Hisao Kamiya

doi:10.2331/fishsci.62.138

Characterization of the Lectin from the Skin Mucus of the Kingklip Genypterus capensis

Michitoshi Toda, Rina Goto-Nance, Koji Muramoto, Hisao Kamiya

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A mitogenic lectin in the skin mucus of the kingklip Genypterus capensis was purified by a combination of affinity adsorption on the glutaraldehyde-fixed rabbit erythrocyte ghosts and chromatofocusing on PBE 94 gels. The lectin was a glycoprotein having a molecular weight of 28-34 kDa which was composed with two homogenous subunit (13.7 kDa). The isoelectric point was 6.45. Kingklip lectin agglutinated rabbit and horse eryttirocytes but not human ABO erythrocytes. The hemagglutinating activity was calcium ion-dependent and was inhibited effectively by asialo-mucin Type I and also by simple sugars such as N-acetyl-D-glucosamine. The amino-terminal sequence of the kingklip lectin was identified as Ser-Met-Cys-Asn-Cys-Gly-Trp-Ser-Gln-Phe-Ala-His-Leu-Ala-Tyr-Leu-Leu-Arg-Ser-Lys- Ala-.

Original language	English
Pages (from-to)	138-141
Number of pages	4
Journal	Fisheries Science
Volume	62
Issue number	1
DOIs	https://doi.org/10.2331/fishsci.62.138
Publication status	Published - 1996 Feb

Keywords

Genypterus capensis
Kingklip
Lectin
Mitogenic
Skin mucus

ASJC Scopus subject areas

Aquatic Science

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10.2331/fishsci.62.138

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title = "Characterization of the Lectin from the Skin Mucus of the Kingklip Genypterus capensis",

abstract = "A mitogenic lectin in the skin mucus of the kingklip Genypterus capensis was purified by a combination of affinity adsorption on the glutaraldehyde-fixed rabbit erythrocyte ghosts and chromatofocusing on PBE 94 gels. The lectin was a glycoprotein having a molecular weight of 28-34 kDa which was composed with two homogenous subunit (13.7 kDa). The isoelectric point was 6.45. Kingklip lectin agglutinated rabbit and horse eryttirocytes but not human ABO erythrocytes. The hemagglutinating activity was calcium ion-dependent and was inhibited effectively by asialo-mucin Type I and also by simple sugars such as N-acetyl-D-glucosamine. The amino-terminal sequence of the kingklip lectin was identified as Ser-Met-Cys-Asn-Cys-Gly-Trp-Ser-Gln-Phe-Ala-His-Leu-Ala-Tyr-Leu-Leu-Arg-Ser-Lys- Ala-.",

keywords = "Genypterus capensis, Kingklip, Lectin, Mitogenic, Skin mucus",

author = "Michitoshi Toda and Rina Goto-Nance and Koji Muramoto and Hisao Kamiya",

year = "1996",

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doi = "10.2331/fishsci.62.138",

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AU - Toda, Michitoshi

AU - Goto-Nance, Rina

AU - Muramoto, Koji

AU - Kamiya, Hisao

PY - 1996/2

Y1 - 1996/2

N2 - A mitogenic lectin in the skin mucus of the kingklip Genypterus capensis was purified by a combination of affinity adsorption on the glutaraldehyde-fixed rabbit erythrocyte ghosts and chromatofocusing on PBE 94 gels. The lectin was a glycoprotein having a molecular weight of 28-34 kDa which was composed with two homogenous subunit (13.7 kDa). The isoelectric point was 6.45. Kingklip lectin agglutinated rabbit and horse eryttirocytes but not human ABO erythrocytes. The hemagglutinating activity was calcium ion-dependent and was inhibited effectively by asialo-mucin Type I and also by simple sugars such as N-acetyl-D-glucosamine. The amino-terminal sequence of the kingklip lectin was identified as Ser-Met-Cys-Asn-Cys-Gly-Trp-Ser-Gln-Phe-Ala-His-Leu-Ala-Tyr-Leu-Leu-Arg-Ser-Lys- Ala-.

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KW - Genypterus capensis

KW - Kingklip

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KW - Mitogenic

KW - Skin mucus

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ER -

Characterization of the Lectin from the Skin Mucus of the Kingklip Genypterus capensis

Abstract

Keywords

ASJC Scopus subject areas

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