TY - JOUR
T1 - Characterization of the Streptococcus mutans pyruvate formate-lyase (PFL)-activating enzyme gene by complementary reconstitution of the in vitro PFL-reactivating system
AU - Yamamoto, Yasuhito
AU - Sato, Yutaka
AU - Takahashi-Abbe, Shoko
AU - Takahashi, Nobuhiro
AU - Kizaki, Harutoshi
PY - 2000
Y1 - 2000
N2 - The act gene was identified and an act mutant as well as the pfl mutant was constructed in Streptococcus mutans. Pyruvate formate-lyase (PFL) activity was regenerated with the mixture of the respective cell extracts from these mutants by complementary reconstitution of the in vitro reactivating system. The S. mutans act gene encoded the sole enzyme able to activate the PFL protein in this organism.
AB - The act gene was identified and an act mutant as well as the pfl mutant was constructed in Streptococcus mutans. Pyruvate formate-lyase (PFL) activity was regenerated with the mixture of the respective cell extracts from these mutants by complementary reconstitution of the in vitro reactivating system. The S. mutans act gene encoded the sole enzyme able to activate the PFL protein in this organism.
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UR - http://www.scopus.com/inward/citedby.url?scp=0033913889&partnerID=8YFLogxK
U2 - 10.1128/IAI.68.8.4773-4777.2000
DO - 10.1128/IAI.68.8.4773-4777.2000
M3 - Article
C2 - 10899886
AN - SCOPUS:0033913889
SN - 0019-9567
VL - 68
SP - 4773
EP - 4777
JO - Infection and Immunity
JF - Infection and Immunity
IS - 8
ER -
