TY - JOUR
T1 - Characterization of TrcMADS1 gene of Trillium camtschatcense (Trilliaceae) reveals functional evolution of the SOC1/TM3-like gene family
AU - Nakamura, Toru
AU - Song, In Ja
AU - Fukuda, Tatsuya
AU - Yokoyama, Jun
AU - Maki, Masayuki
AU - Ochiai, Toshinori
AU - Kameya, Toshiaki
AU - Kanno, Akira
N1 - Funding Information:
Acknowledgements We thank Prof. M. Ohara for kindly providing plant materials. In addition, we thank Mr. H. Tokairin for collaboration in culturing the plants. We are additionally grateful to Y. Ishikawa, P.-Y. Yun, T. Ito, Y. Mashiko, H. Ashizawa, M. Nakada, R. Shinohara, M. Komatsu, S-Y. Kim, and M. Hirai for providing help and advice. This work was supported, in part, by a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology, Japan, and by the Sasakawa Scientific Research Grant from the Japan Science Society.
PY - 2005/7
Y1 - 2005/7
N2 - Plant MADS-box genes encode transcriptional regulators that are critical for a number of developmental processes, such as the establishment of floral organ identity, flowering time, and fruit development. It appears that the MADS-box gene family has undergone considerable gene duplication and divergence within various angiosperm lineages. SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1)/Tomato MADS-box gene 3 (TM3)-like genes are members of the MADS-box gene family and have undergone repeated duplication events. Here, we isolated and characterized the SOC1/TM3-like gene TrcMADS1 from Trillium camtschatcense (Trilliaceae) to infer the ancestral function of SOC1/TM3-like genes. The alignment of SOC1/TM3-like genes revealed the presence of a highly conserved region in the C-terminal of predicted protein sequences, designated the SOC1 motif. Phylogenetic analysis indicated that TrcMADS1 is at the basal position of the SOC1/TM3-like gene family. The TrcMADS1 mRNA was detected in both vegetative and reproductive organs by RT-PCR. Our results suggest that duplicated copies of SOC1/TM3-like gene evolved to become variously functionally specialized.
AB - Plant MADS-box genes encode transcriptional regulators that are critical for a number of developmental processes, such as the establishment of floral organ identity, flowering time, and fruit development. It appears that the MADS-box gene family has undergone considerable gene duplication and divergence within various angiosperm lineages. SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1)/Tomato MADS-box gene 3 (TM3)-like genes are members of the MADS-box gene family and have undergone repeated duplication events. Here, we isolated and characterized the SOC1/TM3-like gene TrcMADS1 from Trillium camtschatcense (Trilliaceae) to infer the ancestral function of SOC1/TM3-like genes. The alignment of SOC1/TM3-like genes revealed the presence of a highly conserved region in the C-terminal of predicted protein sequences, designated the SOC1 motif. Phylogenetic analysis indicated that TrcMADS1 is at the basal position of the SOC1/TM3-like gene family. The TrcMADS1 mRNA was detected in both vegetative and reproductive organs by RT-PCR. Our results suggest that duplicated copies of SOC1/TM3-like gene evolved to become variously functionally specialized.
KW - Gene duplication
KW - Phylogenetic relationship
KW - SOC1 motif
KW - SOC1/TM3-like genes
KW - Subfunctionalization
KW - Trillium camtschatcense
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U2 - 10.1007/s10265-005-0215-5
DO - 10.1007/s10265-005-0215-5
M3 - Article
C2 - 15937720
AN - SCOPUS:23744432902
SN - 0918-9440
VL - 118
SP - 229
EP - 234
JO - Journal of Plant Research
JF - Journal of Plant Research
IS - 3
ER -