TY - JOUR
T1 - Chemerin is a novel regulator of lactogenesis in bovine mammary epithelial cells
AU - Suzuki, Yutaka
AU - Haga, Satoshi
AU - Katoh, Daiki
AU - So, Kyoung Ha
AU - Choi, Ki Choon
AU - Jung, U. Suk
AU - Lee, Hong Gu
AU - Katoh, Kazuo
AU - Roh, Sang Gun
N1 - Funding Information:
This work was supported by JSPS KAKENHI Grant Number 25292158 , 25292163 .
Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.
PY - 2015/10/23
Y1 - 2015/10/23
N2 - Chemerin is a chemoattractant cytokine (chemokine) produced by adipocytes and hepatocytes; it regulates insulin sensitivity and adipocyte differentiation. The objective of this study was to investigate the effect of chemerin on the expression of genes related to lactogenesis and the regulators of chemerin signaling in a bovine mammary epithelial cell line (MAC-T). Two types of chemerin receptors, chemokine like-receptor 1 (CMKLR1) and chemokine (C-C motif) receptor-like 2 (CCRL2), were detected in cultured MAC-T cells, whereas chemerin was not detected. G protein-coupled receptor 1 (GPR1), another receptor of chemerin, was undetectable in MAC-T cells. Chemerin upregulated transcript expression of CMKLR1, CCRL2, and genes associated with fatty acid synthesis, glucose uptake, insulin signaling, and casein synthesis in MAC-T cells. Lactogenic hormones (insulin, growth hormone, and prolactin) downregulated the expression of CMKLR1 in MAC-T cells. Adiponectin suppressed CMKLR1 expression. TNF-α suppressed CMKLR1, but induced CCRL2 expression. These data suggest chemerin is a novel regulator of lactogenesis via its own receptor in bovine mammary epithelial cells.
AB - Chemerin is a chemoattractant cytokine (chemokine) produced by adipocytes and hepatocytes; it regulates insulin sensitivity and adipocyte differentiation. The objective of this study was to investigate the effect of chemerin on the expression of genes related to lactogenesis and the regulators of chemerin signaling in a bovine mammary epithelial cell line (MAC-T). Two types of chemerin receptors, chemokine like-receptor 1 (CMKLR1) and chemokine (C-C motif) receptor-like 2 (CCRL2), were detected in cultured MAC-T cells, whereas chemerin was not detected. G protein-coupled receptor 1 (GPR1), another receptor of chemerin, was undetectable in MAC-T cells. Chemerin upregulated transcript expression of CMKLR1, CCRL2, and genes associated with fatty acid synthesis, glucose uptake, insulin signaling, and casein synthesis in MAC-T cells. Lactogenic hormones (insulin, growth hormone, and prolactin) downregulated the expression of CMKLR1 in MAC-T cells. Adiponectin suppressed CMKLR1 expression. TNF-α suppressed CMKLR1, but induced CCRL2 expression. These data suggest chemerin is a novel regulator of lactogenesis via its own receptor in bovine mammary epithelial cells.
KW - Bovine mammary epithelial cell
KW - Chemerin
KW - Hormonal regulation
KW - Lactogenesis
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U2 - 10.1016/j.bbrc.2015.08.105
DO - 10.1016/j.bbrc.2015.08.105
M3 - Article
C2 - 26342800
AN - SCOPUS:84943365404
SN - 0006-291X
VL - 466
SP - 283
EP - 288
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -