Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system

Jung Min Yoon; Masatoshi Nakajima; Kiyoshi Mashiguchi; Seung Hyun Park; Masato Otani; Tadao Asami

doi:10.1016/j.bmcl.2012.12.007

Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system

Jung Min Yoon, Masatoshi Nakajima, Kiyoshi Mashiguchi, Seung Hyun Park, Masato Otani, Tadao Asami

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins' interaction, a receptor for phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations.

Original language	English
Pages (from-to)	1096-1098
Number of pages	3
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	23
Issue number	4
DOIs	https://doi.org/10.1016/j.bmcl.2012.12.007
Publication status	Published - 2013 Feb 15

Keywords

Gibberellin
Inhibitor
Receptor
Screening
Y2H

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2012.12.007

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title = "Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system",

abstract = "We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins' interaction, a receptor for phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations.",

keywords = "Gibberellin, Inhibitor, Receptor, Screening, Y2H",

author = "Yoon, {Jung Min} and Masatoshi Nakajima and Kiyoshi Mashiguchi and Park, {Seung Hyun} and Masato Otani and Tadao Asami",

note = "Funding Information: We thank Drs. N. Kitahata (Univ. of Tokyo, Japan) and E. Nambara (Univ. of Toronto, Canada) for a kind gift of ABA-related mutants. This work was supported by a grant to M.N. from the Naito Foundation, and by grants to T.A. from the Bio-oriented Technology Research Advancement Institution (PRO-BRAIN) and from Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Agency (JST). ",

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doi = "10.1016/j.bmcl.2012.12.007",

language = "English",

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pages = "1096--1098",

journal = "Bioorganic and Medicinal Chemistry Letters",

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T1 - Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system

AU - Yoon, Jung Min

AU - Nakajima, Masatoshi

AU - Mashiguchi, Kiyoshi

AU - Park, Seung Hyun

AU - Otani, Masato

AU - Asami, Tadao

N1 - Funding Information: We thank Drs. N. Kitahata (Univ. of Tokyo, Japan) and E. Nambara (Univ. of Toronto, Canada) for a kind gift of ABA-related mutants. This work was supported by a grant to M.N. from the Naito Foundation, and by grants to T.A. from the Bio-oriented Technology Research Advancement Institution (PRO-BRAIN) and from Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Agency (JST).

PY - 2013/2/15

Y1 - 2013/2/15

N2 - We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins' interaction, a receptor for phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations.

AB - We applied a yeast two-hybrid (Y2H) system to the high-throughput monitoring of two proteins' interaction, a receptor for phytohormone gibberellin (GA) and its direct signal transducer DELLA. With this system, we screened inhibitors to the interaction. As a result, we discovered a chemical, 3-(2-thienylsulfonyl)pyrazine-2-carbonitrile (TSPC), and we confirmed that TSPC is an inhibitor for GA perception by in vitro and in planta evaluations.

KW - Gibberellin

KW - Inhibitor

KW - Receptor

KW - Screening

KW - Y2H

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JO - Bioorganic and Medicinal Chemistry Letters

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Chemical screening of an inhibitor for gibberellin receptors based on a yeast two-hybrid system

Abstract

Keywords

ASJC Scopus subject areas

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