Chemiluminescence-high performance liquid chromatography of phosphatidylcholine hydroperoxide

Teruo Miyazawa, Keiichi Yasuda, Kenshiro Fujimoto

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165 Citations (Scopus)

Abstract

A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was developed for the hydroperoxide-specific detection of phosphatidylcholine hydroperoxide. In this system, chemiluminescence detector was equipped with normal phase HPLC. Luminol-cytochrome c solution was used as the hydroperoxide-specific luminescent reagent. A detection limit of 7 nmol hydroperoxide-02 of phosphatidylcholine hydroperoxide could be achieved.

Original languageEnglish
Pages (from-to)915-925
Number of pages11
JournalAnalytical Letters
Volume20
Issue number6
DOIs
Publication statusPublished - 1987 Jun

Keywords

  • chemiluminescence
  • HPLC
  • hydroperoxide
  • phosphatidylcholine

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