Abstract
A chemiluminescence-high performance liquid chromatography (CL-HPLC) system was developed for the hydroperoxide-specific detection of phosphatidylcholine hydroperoxide. In this system, chemiluminescence detector was equipped with normal phase HPLC. Luminol-cytochrome c solution was used as the hydroperoxide-specific luminescent reagent. A detection limit of 7 nmol hydroperoxide-02 of phosphatidylcholine hydroperoxide could be achieved.
Original language | English |
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Pages (from-to) | 915-925 |
Number of pages | 11 |
Journal | Analytical Letters |
Volume | 20 |
Issue number | 6 |
DOIs | |
Publication status | Published - 1987 Jun |
Keywords
- chemiluminescence
- HPLC
- hydroperoxide
- phosphatidylcholine