Clock gene mouse period2 overexpression inhibits growth of human pancreatic cancer cells and has synergistic effect with cisplatin

O. D.A. Akira; Y. U. Katayose; Shinichi Yabuuchi; Kuniharu Yamamoto; Masamichi Mizuma; Satoru Shirasou; Toru Onogawa; Hideo Ohtsuka; Hiroshi Yoshida; Hiroki Hayashi; Toshiki Rikiyama; K. I.M. Hyunjung; Youngshik Choe; K. I.M. Kyungjin; S. O.N. Hosun; Fuyuhiko Motoi; Shinichi Egawa; Michiaki Unno

Clock gene mouse period2 overexpression inhibits growth of human pancreatic cancer cells and has synergistic effect with cisplatin

O. D.A. Akira, Y. U. Katayose, Shinichi Yabuuchi, Kuniharu Yamamoto, Masamichi Mizuma, Satoru Shirasou, Toru Onogawa, Hideo Ohtsuka, Hiroshi Yoshida, Hiroki Hayashi, Toshiki Rikiyama, K. I.M. Hyunjung, Youngshik Choe, K. I.M. Kyungjin, S. O.N. Hosun, Fuyuhiko Motoi, Shinichi Egawa, Michiaki Unno

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63 Citations (Scopus)

Abstract

Circadian rhythms are the daily oscillations of multiple biological processes regulated by an endogenous clock. The Period! gene is essential in controlling the circadian rhythm and plays an important role in tumor suppression. We examined whether the overexpression of the mouse Period! gene (mPer2) in cultured tumor cells from human tissues inhibits cell growth, using the recombinant adenovirus vector AdmPer2. The overexpression of mPer2 in human pancreatic cancer cells (Panel, Aspcl) reduced cellular proliferation and induced apoptotic cell death. Infection with AdmPer2 also inhibited cell-cycle progression, inducing arrest at the G ₂-Mphase. Western blotting analyses confirmed that infection with AdmPer2 reduced Bcl-X _L, Cdc2 and cyclin Bl protein, whereas it increased Box protein in Aspcl cells. The overexpression of mPer2 suppressed Cdc2 kinase activity. Moreover, infection with AdmPer2 resulted in.

Original language	English
Pages (from-to)	1201-1210
Number of pages	10
Journal	Anticancer Research
Volume	29
Issue number	4
Publication status	Published - 2009 Apr

Keywords

Apoptosis
Cell cycle arrest
Circadian rhythm
Cisplatin
Clock genes
Mouse period2 gene
Synergic effect
Tumor suppression

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Akira, O. D. A., Katayose, Y. U., Yabuuchi, S., Yamamoto, K., Mizuma, M., Shirasou, S., Onogawa, T., Ohtsuka, H., Yoshida, H., Hayashi, H., Rikiyama, T., Hyunjung, K. I. M., Choe, Y., Kyungjin, K. I. M., Hosun, S. O. N., Motoi, F., Egawa, S., & Unno, M. (2009). Clock gene mouse period2 overexpression inhibits growth of human pancreatic cancer cells and has synergistic effect with cisplatin. Anticancer Research, 29(4), 1201-1210.

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title = "Clock gene mouse period2 overexpression inhibits growth of human pancreatic cancer cells and has synergistic effect with cisplatin",

abstract = "Circadian rhythms are the daily oscillations of multiple biological processes regulated by an endogenous clock. The Period! gene is essential in controlling the circadian rhythm and plays an important role in tumor suppression. We examined whether the overexpression of the mouse Period! gene (mPer2) in cultured tumor cells from human tissues inhibits cell growth, using the recombinant adenovirus vector AdmPer2. The overexpression of mPer2 in human pancreatic cancer cells (Panel, Aspcl) reduced cellular proliferation and induced apoptotic cell death. Infection with AdmPer2 also inhibited cell-cycle progression, inducing arrest at the G 2-Mphase. Western blotting analyses confirmed that infection with AdmPer2 reduced Bcl-X L, Cdc2 and cyclin Bl protein, whereas it increased Box protein in Aspcl cells. The overexpression of mPer2 suppressed Cdc2 kinase activity. Moreover, infection with AdmPer2 resulted in.",

keywords = "Apoptosis, Cell cycle arrest, Circadian rhythm, Cisplatin, Clock genes, Mouse period2 gene, Synergic effect, Tumor suppression",

author = "Akira, {O. D.A.} and Katayose, {Y. U.} and Shinichi Yabuuchi and Kuniharu Yamamoto and Masamichi Mizuma and Satoru Shirasou and Toru Onogawa and Hideo Ohtsuka and Hiroshi Yoshida and Hiroki Hayashi and Toshiki Rikiyama and Hyunjung, {K. I.M.} and Youngshik Choe and Kyungjin, {K. I.M.} and Hosun, {S. O.N.} and Fuyuhiko Motoi and Shinichi Egawa and Michiaki Unno",

year = "2009",

month = apr,

language = "English",

volume = "29",

pages = "1201--1210",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "4",

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Akira, ODA, Katayose, YU, Yabuuchi, S, Yamamoto, K, Mizuma, M, Shirasou, S, Onogawa, T, Ohtsuka, H, Yoshida, H, Hayashi, H, Rikiyama, T, Hyunjung, KIM, Choe, Y, Kyungjin, KIM, Hosun, SON, Motoi, F, Egawa, S & Unno, M 2009, 'Clock gene mouse period2 overexpression inhibits growth of human pancreatic cancer cells and has synergistic effect with cisplatin', Anticancer Research, vol. 29, no. 4, pp. 1201-1210.

TY - JOUR

T1 - Clock gene mouse period2 overexpression inhibits growth of human pancreatic cancer cells and has synergistic effect with cisplatin

AU - Akira, O. D.A.

AU - Katayose, Y. U.

AU - Yabuuchi, Shinichi

AU - Yamamoto, Kuniharu

AU - Mizuma, Masamichi

AU - Shirasou, Satoru

AU - Onogawa, Toru

AU - Ohtsuka, Hideo

AU - Yoshida, Hiroshi

AU - Hayashi, Hiroki

AU - Rikiyama, Toshiki

AU - Hyunjung, K. I.M.

AU - Choe, Youngshik

AU - Kyungjin, K. I.M.

AU - Hosun, S. O.N.

AU - Motoi, Fuyuhiko

AU - Egawa, Shinichi

AU - Unno, Michiaki

PY - 2009/4

Y1 - 2009/4

N2 - Circadian rhythms are the daily oscillations of multiple biological processes regulated by an endogenous clock. The Period! gene is essential in controlling the circadian rhythm and plays an important role in tumor suppression. We examined whether the overexpression of the mouse Period! gene (mPer2) in cultured tumor cells from human tissues inhibits cell growth, using the recombinant adenovirus vector AdmPer2. The overexpression of mPer2 in human pancreatic cancer cells (Panel, Aspcl) reduced cellular proliferation and induced apoptotic cell death. Infection with AdmPer2 also inhibited cell-cycle progression, inducing arrest at the G 2-Mphase. Western blotting analyses confirmed that infection with AdmPer2 reduced Bcl-X L, Cdc2 and cyclin Bl protein, whereas it increased Box protein in Aspcl cells. The overexpression of mPer2 suppressed Cdc2 kinase activity. Moreover, infection with AdmPer2 resulted in.

AB - Circadian rhythms are the daily oscillations of multiple biological processes regulated by an endogenous clock. The Period! gene is essential in controlling the circadian rhythm and plays an important role in tumor suppression. We examined whether the overexpression of the mouse Period! gene (mPer2) in cultured tumor cells from human tissues inhibits cell growth, using the recombinant adenovirus vector AdmPer2. The overexpression of mPer2 in human pancreatic cancer cells (Panel, Aspcl) reduced cellular proliferation and induced apoptotic cell death. Infection with AdmPer2 also inhibited cell-cycle progression, inducing arrest at the G 2-Mphase. Western blotting analyses confirmed that infection with AdmPer2 reduced Bcl-X L, Cdc2 and cyclin Bl protein, whereas it increased Box protein in Aspcl cells. The overexpression of mPer2 suppressed Cdc2 kinase activity. Moreover, infection with AdmPer2 resulted in.

KW - Apoptosis

KW - Cell cycle arrest

KW - Circadian rhythm

KW - Cisplatin

KW - Clock genes

KW - Mouse period2 gene

KW - Synergic effect

KW - Tumor suppression

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AN - SCOPUS:64949157835

SN - 0250-7005

VL - 29

SP - 1201

EP - 1210

JO - Anticancer Research

JF - Anticancer Research

IS - 4

ER -

Clock gene mouse period2 overexpression inhibits growth of human pancreatic cancer cells and has synergistic effect with cisplatin

Abstract

Keywords

UN SDGs

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