TY - JOUR
T1 - Cloning and characterization of a third type of human α-amylase gene, AMY2B
AU - Yokouchi, Hideoki
AU - Horii, Akira
AU - Emi, Mitsuru
AU - Tomita, Naohiro
AU - Doi, Sadayuki
AU - Ogawa, Michio
AU - Mori, Takesada
AU - Matsubara, Kenichi
N1 - Funding Information:
We thank Dr. Y. Nakamura for providing us with the pJB8-based human genomic cosmid library, and Miss K. Mimura for her secretarial assistance and preparation of the manuscript. This work was supported by a Scientific Research Grant to K.M. from the Ministry of Education, Science and Culture, Japan.
PY - 1990
Y1 - 1990
N2 - We have previously reported concerning the existence of a third type of human α-amylase gene, AMY3 [Emi et al., Gene 62 (1988) 229-235; Tomita et al., Gene 76 (1989) 11-18], which is expressed in a lung carcinoid tissue, and differs in nucleotide sequence from the two previously characterized human α-amylase genes coding for salivary and pancreatic isozymes, termed AMY1 and AMY2, respectively. Here, we rename this gene AMY2B to coincide with the designation by Gumucio et al. [Mol. Cell Biol. 8 (1988) 1197-1205] and describe its genetic properties as revealed by sequencing studies. It consists of ten major exons whose sequences are highly homologous to those of AMY1 and AMY2. Not only the exons, but also most of the introns seem to be highly conserved, as judged from physical mapping data. The AMY2B gene identified from mRNA in a lung carcinoid tissue has at least two additional untranslated exons in its 5′ region; hence the promoter lies far upstream relative to the other two AMY genes.
AB - We have previously reported concerning the existence of a third type of human α-amylase gene, AMY3 [Emi et al., Gene 62 (1988) 229-235; Tomita et al., Gene 76 (1989) 11-18], which is expressed in a lung carcinoid tissue, and differs in nucleotide sequence from the two previously characterized human α-amylase genes coding for salivary and pancreatic isozymes, termed AMY1 and AMY2, respectively. Here, we rename this gene AMY2B to coincide with the designation by Gumucio et al. [Mol. Cell Biol. 8 (1988) 1197-1205] and describe its genetic properties as revealed by sequencing studies. It consists of ten major exons whose sequences are highly homologous to those of AMY1 and AMY2. Not only the exons, but also most of the introns seem to be highly conserved, as judged from physical mapping data. The AMY2B gene identified from mRNA in a lung carcinoid tissue has at least two additional untranslated exons in its 5′ region; hence the promoter lies far upstream relative to the other two AMY genes.
KW - Recombinant DNA
KW - amylase isozyme
KW - cosmid
KW - exon
KW - genomic library
KW - intron
KW - lung carcinoid tumor
KW - multi-gene family
KW - processed pseudogene
KW - γ-actin
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U2 - 10.1016/0378-1119(90)90191-S
DO - 10.1016/0378-1119(90)90191-S
M3 - Article
C2 - 2401405
AN - SCOPUS:0025025819
SN - 0378-1119
VL - 90
SP - 281
EP - 286
JO - Gene
JF - Gene
IS - 2
ER -