TY - JOUR
T1 - Cloning and expression of the gene encoding solanesyl diphosphate synthase from Hevea brasiliensis
AU - Phatthiya, Atiphon
AU - Takahashi, Seiji
AU - Chareonthiphakorn, Nopphakaew
AU - Koyama, Tanetoshi
AU - Wititsuwannakul, Dhirayos
AU - Wititsuwannakul, Rapepun
N1 - Funding Information:
This work was supported by grants from Thailand Research Fund, TRF Royal Golden Jubilee-Ph.D. Program, Association of International Education, Japan, and Prince of Songkla University. We thank Dr. Brian Hodgson for the critical reading of the manuscript.
PY - 2007/4
Y1 - 2007/4
N2 - Plastoquinones play important roles as electron carriers in the light-dependent reactions of photosynthesis and also as a cofactor of phytoene desaturation in the synthesis of carotenoid. A plastoquinone-9 (PQ-9) was identified in Frey-Wyssling organelles of fresh rubber latex from Hevea brasiliensis. This indicates that a Hevea solanesyl diphosphate synthase (HbSDS) must be present for the synthesis of the C45 prenyl side chain of the PQ-9 found in the rubber latex. Based on the sequence information of other trans-prenyl diphosphate synthases, the cDNA encoding HbSDS was cloned from a Hevea rubber latex cDNA library. Sequence analysis revealed the presence of an ORF consisting of 1254 bp capable of encoding a 46,095 Da polypeptide. The deduced amino acid sequence of the clone contained all conserved regions found in typical trans-prenyl chain elongating enzymes. This cDNA, expressed in Escherichia coli cells as an insoluble but Trx-His-tagged fusion protein, showed a distinct solanesyl diphosphate synthase activity in the presence of Triton X-100. The expression level of HbSDS mRNA in latex was found to be higher than those in leaves, stems and roots. The expressed HbSDS is suggested to be a specific enzyme in the Frey-Wyssling particles of rubber latex.
AB - Plastoquinones play important roles as electron carriers in the light-dependent reactions of photosynthesis and also as a cofactor of phytoene desaturation in the synthesis of carotenoid. A plastoquinone-9 (PQ-9) was identified in Frey-Wyssling organelles of fresh rubber latex from Hevea brasiliensis. This indicates that a Hevea solanesyl diphosphate synthase (HbSDS) must be present for the synthesis of the C45 prenyl side chain of the PQ-9 found in the rubber latex. Based on the sequence information of other trans-prenyl diphosphate synthases, the cDNA encoding HbSDS was cloned from a Hevea rubber latex cDNA library. Sequence analysis revealed the presence of an ORF consisting of 1254 bp capable of encoding a 46,095 Da polypeptide. The deduced amino acid sequence of the clone contained all conserved regions found in typical trans-prenyl chain elongating enzymes. This cDNA, expressed in Escherichia coli cells as an insoluble but Trx-His-tagged fusion protein, showed a distinct solanesyl diphosphate synthase activity in the presence of Triton X-100. The expression level of HbSDS mRNA in latex was found to be higher than those in leaves, stems and roots. The expressed HbSDS is suggested to be a specific enzyme in the Frey-Wyssling particles of rubber latex.
KW - Frey-Wyssling particle
KW - Hevea brasiliensis
KW - Plastoquinone
KW - Prenyl diphosphate synthase
KW - Rubber latex
KW - Solanesyl diphosphate synthase
UR - http://www.scopus.com/inward/record.url?scp=33847284023&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847284023&partnerID=8YFLogxK
U2 - 10.1016/j.plantsci.2006.12.015
DO - 10.1016/j.plantsci.2006.12.015
M3 - Article
AN - SCOPUS:33847284023
SN - 0168-9452
VL - 172
SP - 824
EP - 831
JO - Plant Science
JF - Plant Science
IS - 4
ER -