Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca

Takanori Ambo, Motoyoshi Noike, Hirofumi Kurokawa, Tanetoshi Koyama

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

cis-Prenyltransferase catalyzes the synthesis of Z,E-mixed prenyl diphosphates by a condensation of isopentenyl diphosphate to an allylic diphosphate. A novel gene encoding a cis-prenyltransferase is cloned from Thermobifida fusca. It showed a unique substrate specificity accepting dimethylallyl diphosphate as a shortest allylic substrate, and synthesizes polyprenyl products up to C70.

Original languageEnglish
Pages (from-to)620-622
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume107
Issue number6
DOIs
Publication statusPublished - 2009 Jun

Keywords

  • Actinomycete
  • Isoprenoid biosynthesis
  • Prenyltransferase
  • Sugar carrier lipid biosynthesis
  • Thermobifida fusca

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