TY - JOUR
T1 - Cloning and genomic characterization of sytdep, a new synaptotagmin XIV-related gene
AU - Herrero-Turrión, M. Javier
AU - Fukuda, Mitsunori
AU - Mollinedo, Faustino
N1 - Funding Information:
We are indebted to Javier de la Riva and Alberto de Luis for their assistance with the BLAST programs. We thank the Blood Bank of the Hospital Universitario of Salamanca for blood supply. This work was supported by grants from the Fondo de Investigación Sanitaria and European Commission (FIS-FEDER 04/0843, 02/1199, and 01/1048), Ministerio de Educación y Ciencia (SAF2005-04293), Fundación de Investigación Médica Mutua Madrileña (FMM), Fundación “la Caixa” (BM05-30-0), and Junta de Castilla y León (CSI04A05).
PY - 2006/2/10
Y1 - 2006/2/10
N2 - We have identified a new human gene coined sytdep (synaptotagmin XIV-derived protein) in human neutrophils. Sytdep encodes a 188-amino acid sequence with a 21.435 kDa deduced molecular mass, showing 75% identity to human synaptotagmin (syt) XIV. Human neutrophils express sytdep, but not syt XIV. Sytdep was upregulated during HL-60 neutrophil differentiation. Sytdep gene is located in human chromosome 4 and contains a unique exon, whereas syt XIV gene, located in chromosome 1, comprises 10 exons with 9 introns. Mouse genome did not contain sytdep. The N-terminal region of sytdep shows no homology with any known protein and, unlike synaptotagmin XIV isoforms, sytdep shows a unique C-terminal C2B domain. Polyclonal antibodies against the C2B domain of syt XIV recognized sytdep as a 27-kDa protein in human neutrophils. Genomic analyses suggest that human sytdep could derive from a retrotranslocation of a syt XIV transcript into chromosome 4.
AB - We have identified a new human gene coined sytdep (synaptotagmin XIV-derived protein) in human neutrophils. Sytdep encodes a 188-amino acid sequence with a 21.435 kDa deduced molecular mass, showing 75% identity to human synaptotagmin (syt) XIV. Human neutrophils express sytdep, but not syt XIV. Sytdep was upregulated during HL-60 neutrophil differentiation. Sytdep gene is located in human chromosome 4 and contains a unique exon, whereas syt XIV gene, located in chromosome 1, comprises 10 exons with 9 introns. Mouse genome did not contain sytdep. The N-terminal region of sytdep shows no homology with any known protein and, unlike synaptotagmin XIV isoforms, sytdep shows a unique C-terminal C2B domain. Polyclonal antibodies against the C2B domain of syt XIV recognized sytdep as a 27-kDa protein in human neutrophils. Genomic analyses suggest that human sytdep could derive from a retrotranslocation of a syt XIV transcript into chromosome 4.
KW - Alternative splicing
KW - C2 domain
KW - Cloning
KW - Gene expression
KW - Genomic characterization
KW - Human neutrophils
KW - Synaptotagmin XIV-derived gene
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U2 - 10.1016/j.bbrc.2005.11.184
DO - 10.1016/j.bbrc.2005.11.184
M3 - Article
C2 - 16376304
AN - SCOPUS:29644442950
SN - 0006-291X
VL - 340
SP - 386
EP - 394
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -