Cloning, heterologous expression, renaturation, and characterization of a cold-adapted esterase with unique primary structure from a psychrotroph Pseudomonas sp. strain B11-1

Biochemistry, Genetics and Molecular Biology

• Lysozyme 100%
• Renaturation 60%
• Peptide Sequence 40%
• Urea 40%
• Pseudomonas 40%
• Protein 40%
• Nested Gene 20%
• Enzymatic Hydrolysis 20%
• Lipase 20%
• Escherichia coli 20%
• Bacterium 20%
• Molecular Weight 20%
• Heterologous Expression 20%
• Energy 20%
• High Temperature 20%
• Dialysis 20%
• Hydrolase 20%
• Nucleophilicity 20%
• Catalytic Triad 20%
• T7 RNA Polymerase 20%
• Sequence Motif 20%
• Growth 20%
• Temperature 20%
• Length 20%
• Transcription 20%
• Coding 20%
• Amino Acids 20%
• Identity 20%