Cloning of γ-hexachlorocyclohexane dehydrochlorinase gene with its flanking regions from soil by activity-based screening techniques

Michihiro Ito; Akira Ono; Yoshiyuki Ohtsubo; Masataka Tsuda; Yuji Nagata

doi:10.1016/j.ejsobi.2012.05.001

Cloning of γ-hexachlorocyclohexane dehydrochlorinase gene with its flanking regions from soil by activity-based screening techniques

Michihiro Ito, Akira Ono, Yoshiyuki Ohtsubo, Masataka Tsuda, Yuji Nagata

LIF - Molecular and Chemical Life Science

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Activity-based screening techniques were applied to clone a gene encoding γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase with its flanking regions from a cosmid-based library of DNA that was extracted from a γ-HCH-added suspension of HCH-contaminated soil. A total of 11 cosmid clones showing the γ-HCH dehydrochlorinase activity were obtained through the screenings, indicating the validity of this approach. All the clones had a linA gene identical to known one, but its flanking regions showed some structural variations with known ones, suggesting high likelihood of genetic divergence in the linA-flanking regions.

Original language	English
Pages (from-to)	16-19
Number of pages	4
Journal	European Journal of Soil Biology
Volume	52
DOIs	https://doi.org/10.1016/j.ejsobi.2012.05.001
Publication status	Published - 2012 Sept

Keywords

γ-Hexachlorocyclohexane
Biodegradation
Dehalogenase
Dehydrochlorinase
Xenobiotics

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10.1016/j.ejsobi.2012.05.001

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title = "Cloning of γ-hexachlorocyclohexane dehydrochlorinase gene with its flanking regions from soil by activity-based screening techniques",

abstract = "Activity-based screening techniques were applied to clone a gene encoding γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase with its flanking regions from a cosmid-based library of DNA that was extracted from a γ-HCH-added suspension of HCH-contaminated soil. A total of 11 cosmid clones showing the γ-HCH dehydrochlorinase activity were obtained through the screenings, indicating the validity of this approach. All the clones had a linA gene identical to known one, but its flanking regions showed some structural variations with known ones, suggesting high likelihood of genetic divergence in the linA-flanking regions.",

keywords = "γ-Hexachlorocyclohexane, Biodegradation, Dehalogenase, Dehydrochlorinase, Xenobiotics",

author = "Michihiro Ito and Akira Ono and Yoshiyuki Ohtsubo and Masataka Tsuda and Yuji Nagata",

note = "Funding Information: This work was supported in part by Grant-in-Aids for Scientific Research from Ministry of Education, Culture, Sports, Science, and Technology , and Ministry of Agriculture, Forestry, and Fisheries of Japan .",

year = "2012",

month = sep,

doi = "10.1016/j.ejsobi.2012.05.001",

language = "English",

volume = "52",

pages = "16--19",

journal = "European Journal of Soil Biology",

issn = "1164-5563",

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T1 - Cloning of γ-hexachlorocyclohexane dehydrochlorinase gene with its flanking regions from soil by activity-based screening techniques

AU - Ito, Michihiro

AU - Ono, Akira

AU - Ohtsubo, Yoshiyuki

AU - Tsuda, Masataka

AU - Nagata, Yuji

N1 - Funding Information: This work was supported in part by Grant-in-Aids for Scientific Research from Ministry of Education, Culture, Sports, Science, and Technology , and Ministry of Agriculture, Forestry, and Fisheries of Japan .

PY - 2012/9

Y1 - 2012/9

N2 - Activity-based screening techniques were applied to clone a gene encoding γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase with its flanking regions from a cosmid-based library of DNA that was extracted from a γ-HCH-added suspension of HCH-contaminated soil. A total of 11 cosmid clones showing the γ-HCH dehydrochlorinase activity were obtained through the screenings, indicating the validity of this approach. All the clones had a linA gene identical to known one, but its flanking regions showed some structural variations with known ones, suggesting high likelihood of genetic divergence in the linA-flanking regions.

AB - Activity-based screening techniques were applied to clone a gene encoding γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase with its flanking regions from a cosmid-based library of DNA that was extracted from a γ-HCH-added suspension of HCH-contaminated soil. A total of 11 cosmid clones showing the γ-HCH dehydrochlorinase activity were obtained through the screenings, indicating the validity of this approach. All the clones had a linA gene identical to known one, but its flanking regions showed some structural variations with known ones, suggesting high likelihood of genetic divergence in the linA-flanking regions.

KW - γ-Hexachlorocyclohexane

KW - Biodegradation

KW - Dehalogenase

KW - Dehydrochlorinase

KW - Xenobiotics

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DO - 10.1016/j.ejsobi.2012.05.001

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AN - SCOPUS:84861825740

SN - 1164-5563

VL - 52

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JO - European Journal of Soil Biology

JF - European Journal of Soil Biology

ER -

Cloning of γ-hexachlorocyclohexane dehydrochlorinase gene with its flanking regions from soil by activity-based screening techniques

Abstract

Keywords

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