Co-localization of glucokinase with actin filaments

Tomiyasu Murata; Hideki Katagiri; Hisamitsu Ishihara; Yoshikazu Shibasaki; Tomoichiro Asano; Yukiyasu Toyoda; Bilgehan Pekiner; Can Pekiner; Ichitomo Miwa; Yoshitomo Oka

doi:10.1016/S0014-5793(97)00253-6

Co-localization of glucokinase with actin filaments

Tomiyasu Murata, Hideki Katagiri, Hisamitsu Ishihara, Yoshikazu Shibasaki, Tomoichiro Asano, Yukiyasu Toyoda, Bilgehan Pekiner, Can Pekiner, Ichitomo Miwa, Yoshitomo Oka

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

A portion of glucokinase appeared to be co-localized with actin filaments in the cytoplasm of cultured rat hepatocytes incubated with 25 mM glucose. When liver- or islet-type glucokinase was transiently expressed in COS-7 cells, the expressed glucokinase was also co-localized with actin filaments in the cytoplasm of these transfected cells. Although co-localization of glucokinase with actin filaments was not clearly demonstrated in the pancreatic β-cell line MIN6, islet glucokinase was found to be present in both the nucleus and the cytoplasm, though predominantly in the nucleus. These findings suggest that subcellular localization of glucokinase, including co-localization with actin filaments, may have an important physiological role in metabolic regulation.

Original language	English
Pages (from-to)	109-113
Number of pages	5
Journal	FEBS Letters
Volume	406
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(97)00253-6
Publication status	Published - 1997 Apr 7

Keywords

Actin filament
Islet glucokinase
Liver glucokinase

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10.1016/S0014-5793(97)00253-6

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@article{0393254d6a9f461d930413db19174155,

title = "Co-localization of glucokinase with actin filaments",

abstract = "A portion of glucokinase appeared to be co-localized with actin filaments in the cytoplasm of cultured rat hepatocytes incubated with 25 mM glucose. When liver- or islet-type glucokinase was transiently expressed in COS-7 cells, the expressed glucokinase was also co-localized with actin filaments in the cytoplasm of these transfected cells. Although co-localization of glucokinase with actin filaments was not clearly demonstrated in the pancreatic β-cell line MIN6, islet glucokinase was found to be present in both the nucleus and the cytoplasm, though predominantly in the nucleus. These findings suggest that subcellular localization of glucokinase, including co-localization with actin filaments, may have an important physiological role in metabolic regulation.",

keywords = "Actin filament, Islet glucokinase, Liver glucokinase",

author = "Tomiyasu Murata and Hideki Katagiri and Hisamitsu Ishihara and Yoshikazu Shibasaki and Tomoichiro Asano and Yukiyasu Toyoda and Bilgehan Pekiner and Can Pekiner and Ichitomo Miwa and Yoshitomo Oka",

note = "Funding Information: This investigation was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (08457266) and a grant from Uehara Memorial Foundation to Y.O.",

year = "1997",

month = apr,

day = "7",

doi = "10.1016/S0014-5793(97)00253-6",

language = "English",

volume = "406",

pages = "109--113",

journal = "FEBS Letters",

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TY - JOUR

T1 - Co-localization of glucokinase with actin filaments

AU - Murata, Tomiyasu

AU - Katagiri, Hideki

AU - Ishihara, Hisamitsu

AU - Shibasaki, Yoshikazu

AU - Asano, Tomoichiro

AU - Toyoda, Yukiyasu

AU - Pekiner, Bilgehan

AU - Pekiner, Can

AU - Miwa, Ichitomo

AU - Oka, Yoshitomo

N1 - Funding Information: This investigation was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (08457266) and a grant from Uehara Memorial Foundation to Y.O.

PY - 1997/4/7

Y1 - 1997/4/7

N2 - A portion of glucokinase appeared to be co-localized with actin filaments in the cytoplasm of cultured rat hepatocytes incubated with 25 mM glucose. When liver- or islet-type glucokinase was transiently expressed in COS-7 cells, the expressed glucokinase was also co-localized with actin filaments in the cytoplasm of these transfected cells. Although co-localization of glucokinase with actin filaments was not clearly demonstrated in the pancreatic β-cell line MIN6, islet glucokinase was found to be present in both the nucleus and the cytoplasm, though predominantly in the nucleus. These findings suggest that subcellular localization of glucokinase, including co-localization with actin filaments, may have an important physiological role in metabolic regulation.

AB - A portion of glucokinase appeared to be co-localized with actin filaments in the cytoplasm of cultured rat hepatocytes incubated with 25 mM glucose. When liver- or islet-type glucokinase was transiently expressed in COS-7 cells, the expressed glucokinase was also co-localized with actin filaments in the cytoplasm of these transfected cells. Although co-localization of glucokinase with actin filaments was not clearly demonstrated in the pancreatic β-cell line MIN6, islet glucokinase was found to be present in both the nucleus and the cytoplasm, though predominantly in the nucleus. These findings suggest that subcellular localization of glucokinase, including co-localization with actin filaments, may have an important physiological role in metabolic regulation.

KW - Actin filament

KW - Islet glucokinase

KW - Liver glucokinase

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U2 - 10.1016/S0014-5793(97)00253-6

DO - 10.1016/S0014-5793(97)00253-6

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C2 - 9109397

AN - SCOPUS:0031006435

SN - 0014-5793

VL - 406

SP - 109

EP - 113

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Co-localization of glucokinase with actin filaments

Abstract

Keywords

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