Cold atmospheric plasma enhances osteoblast differentiation

Kanako Tominami; Hiroyasu Kanetaka; Shota Sasaki; Takayuki Mokudai; Toshiro Kaneko; Yoshimi Niwano

doi:10.1371/journal.pone.0180507

Cold atmospheric plasma enhances osteoblast differentiation

Kanako Tominami, Hiroyasu Kanetaka, Shota Sasaki, Takayuki Mokudai, Toshiro Kaneko, Yoshimi Niwano

Tohoku University

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining were applied to assess osteoblastic differentiation. The plasma-generated radicals were detected directly using an electron spin resonance-spin trapping technique. Results show that plasma irradiation under specific conditions increased ALP activity and enhanced mineralization, and demonstrated that the yield of radicals was increased in an irradiation-time-dependent manner. Appropriate plasma irradiation stimulated the osteoblastic differentiation of the cells. This process offers the potential of promoting bone regeneration.

Original language	English
Article number	e0180507
Journal	PLoS ONE
Volume	12
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0180507
Publication status	Published - 2017 Jul

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10.1371/journal.pone.0180507

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AU - Sasaki, Shota

AU - Mokudai, Takayuki

AU - Kaneko, Toshiro

AU - Niwano, Yoshimi

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Cold atmospheric plasma enhances osteoblast differentiation

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