TY - JOUR
T1 - Collagen immobilization on 316L stainless steel surface with cathodic deposition of calcium phosphate
AU - Roguska, Agata
AU - Hiromoto, Sachiko
AU - Yamamoto, Akiko
AU - Woźniak, Michał Jerzy
AU - Pisarek, Marcin
AU - Lewandowska, Małgorzata
N1 - Funding Information:
The authors acknowledge the WUT-NIMS Joint Graduate School Program for the NIMS Junior Researcher Fellowship for A. Roguska. Special thanks are dedicated to Y. Kohyama for her help and advice to A. Roguska on the cell culture experiments.
PY - 2011/3/15
Y1 - 2011/3/15
N2 - Collagen fibril/(calcium phosphate and carbonate) composite coatings on 316L stainless steel were developed with a cathodic deposition technique. The response of SaOS-2 osteoblast-like cells to the collagen/calcium salt-coated 316L steel was investigated. The collagen fibrils were self-assembled on the 316L steel surface and immobilized by their partial incorporation into a calcium salt layer electrodeposited cathodically in Hanks' solution. The amount of calcium salt depended on the applied cathodic potential. The mineralization of collagen fibrils was observed. The collagen coverage localized and the composition of calcium salts varied on the same specimen. Such non-uniform surfaces affected the cell response. The observed outlines of cell bodies and nuclei on the thin collagen coating were clearer than those on the thick collagen coating in most cases. The collagen coating did not significantly influence the mean viability of cells on the whole specimen surface. Interestingly, the alkaline phosphatase activity per cell on the collagen/calcium salt-coated specimens was higher than that on the as-received specimen. It was revealed that cathodic deposition is an effective technique to immobilize collagen fibrils on a 316L steel surface.
AB - Collagen fibril/(calcium phosphate and carbonate) composite coatings on 316L stainless steel were developed with a cathodic deposition technique. The response of SaOS-2 osteoblast-like cells to the collagen/calcium salt-coated 316L steel was investigated. The collagen fibrils were self-assembled on the 316L steel surface and immobilized by their partial incorporation into a calcium salt layer electrodeposited cathodically in Hanks' solution. The amount of calcium salt depended on the applied cathodic potential. The mineralization of collagen fibrils was observed. The collagen coverage localized and the composition of calcium salts varied on the same specimen. Such non-uniform surfaces affected the cell response. The observed outlines of cell bodies and nuclei on the thin collagen coating were clearer than those on the thick collagen coating in most cases. The collagen coating did not significantly influence the mean viability of cells on the whole specimen surface. Interestingly, the alkaline phosphatase activity per cell on the collagen/calcium salt-coated specimens was higher than that on the as-received specimen. It was revealed that cathodic deposition is an effective technique to immobilize collagen fibrils on a 316L steel surface.
KW - 316L stainless steel
KW - Calcium carbonate
KW - Calcium phosphate
KW - Collagen
KW - Electrochemical treatment
KW - SaOS-2 osteoblast-like cells
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U2 - 10.1016/j.apsusc.2011.01.018
DO - 10.1016/j.apsusc.2011.01.018
M3 - Article
AN - SCOPUS:79951680691
SN - 0169-4332
VL - 257
SP - 5037
EP - 5045
JO - Applied Surface Science
JF - Applied Surface Science
IS - 11
ER -