Abstract
Fish skin can be extracted and hydrolyzed into collagen peptide to enhance the antioxidant activity. The research objectives were to determine the characteristics of collagen peptides from pangasius fish skin as antioxidants. The fish skin was extracted and hydrolyzed using 2% Alcalase and fractionated using a molecular sieve membrane. The gelatin yield was 15.07±1.45% against the starting material. The pH value of gelatin was 4.76±0.02. SDS-PAGE showed β band 221.3 kDa, α1 band 137.1 kDa and α2 band 117.7 kDa. The hydrolysis of gelatin resulted in the degree of hydrolysis of 48.06±1.97% with Alcalase at 55°C for 180 min. The total antioxidant activity (TEAC) was calculated with ABTS assays. The TEAC values were 24.53±0.78 μmol TE/g for the gelatin, 44.40±0.13 μmol TE/g for the peptides, 45.07±0.12 μmol TE/g for the >30 kDa fraction, 45.85±0.04 μmol TE/g for the 10-30 kDa fraction, 45.93±0.04 μmol TE/g for the 3-10 kDa fraction, and 45.98±0.04 μmol TE/g for the <3 kDa fraction.
| Original language | English |
|---|---|
| Article number | 012055 |
| Journal | IOP Conference Series: Earth and Environmental Science |
| Volume | 404 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2019 Dec 19 |
| Event | 4th EMBRIO International Symposium, EIS 2019 and the 7th International Symposium of East Asia Fisheries and Technologists Association, EAFTA 2019 - Bogor, Indonesia Duration: 2019 Aug 5 → 2019 Aug 6 |
Keywords
- antioxidant
- collagen peptide
- extraction
- fractionation
- hydrolysis
ASJC Scopus subject areas
- Environmental Science(all)
- Earth and Planetary Sciences(all)