TY - JOUR
T1 - Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells
AU - Tsuchiya, S.
AU - Honda, M. J.
AU - Shinohara, Y.
AU - Saito, M.
AU - Ueda, M.
N1 - Funding Information:
This work was supported in part by grants from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (Kakenhi Kiban B 16390578 and Houga 18659592 to M.J.H.) and by the Hitachi Medical Corporation (Japan) and DENICS International (Japan). S.Tsuchiya.M.J.Honda(*).Y.Shinohara.M.Ueda Tooth Regeneration, The Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shiroganedai, Minato-ku, Tokyo 108-8639, Japan e-mail: honda-m@ims.u-tokyo.ac.jp
PY - 2008/2
Y1 - 2008/2
N2 - We investigated porcine dental follicle cells at the early crown-formation stage and examined the behavior of cells grown in a collagen type I (Col-I) matrix. Clone-porcine dental follicle cells (DFC-I) and controls, viz., dental follicle itself, nonclone-dental follicle cells, periodontal ligament cells (PDLC), and bone marrow stromal cells, were obtained from 6-month-old pigs. DFC-I showed a different gene expression pattern from controls by reverse-transcription polymerase chain reaction analysis. In addition, Col-I treatment enhanced DFC-I proliferation and increased their alkaline phosphatase activity compared with nontreated DFC-I. The expression of periostin, biglycan, and osteocalcin (OCN) in cells growing on collagen was upregulated, similar to the pattern seen in PDLC. DFC-I with and without Col-I treatment were combined with β-tricalcium phosphate particles and implanted into immunodeficient mice. Significant differences were found in the gene expression patterns of bone sialoprotein, OCN, and periostin in both treated and non-treated implants at 2 and/or 4 weeks. The results showed that Col-I induced the mineralization pathway in these cells. Hard tissue formation was observed in both implant types at 8 weeks. Our results suggest that Col-I facilitates the differentiation of DFC-I along the mineralization process.
AB - We investigated porcine dental follicle cells at the early crown-formation stage and examined the behavior of cells grown in a collagen type I (Col-I) matrix. Clone-porcine dental follicle cells (DFC-I) and controls, viz., dental follicle itself, nonclone-dental follicle cells, periodontal ligament cells (PDLC), and bone marrow stromal cells, were obtained from 6-month-old pigs. DFC-I showed a different gene expression pattern from controls by reverse-transcription polymerase chain reaction analysis. In addition, Col-I treatment enhanced DFC-I proliferation and increased their alkaline phosphatase activity compared with nontreated DFC-I. The expression of periostin, biglycan, and osteocalcin (OCN) in cells growing on collagen was upregulated, similar to the pattern seen in PDLC. DFC-I with and without Col-I treatment were combined with β-tricalcium phosphate particles and implanted into immunodeficient mice. Significant differences were found in the gene expression patterns of bone sialoprotein, OCN, and periostin in both treated and non-treated implants at 2 and/or 4 weeks. The results showed that Col-I induced the mineralization pathway in these cells. Hard tissue formation was observed in both implant types at 8 weeks. Our results suggest that Col-I facilitates the differentiation of DFC-I along the mineralization process.
KW - Characterization
KW - Clonal dental follicle cell
KW - Collagen type I matrix
KW - Dental follicle
KW - Mineralization
KW - Porcine
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U2 - 10.1007/s00441-007-0532-1
DO - 10.1007/s00441-007-0532-1
M3 - Article
C2 - 17999085
AN - SCOPUS:38349029319
SN - 0302-766X
VL - 331
SP - 447
EP - 459
JO - Cell and Tissue Research
JF - Cell and Tissue Research
IS - 2
ER -