Collection of biochemical samples with brain-wide electrophysiological recordings from a freely moving rodent

Daichi Konno, Ryota Nakayama, Makoto Tsunoda, Takashi Funatsu, Yuji Ikegaya, Takuya Sasaki

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Bridging accumulating insights from microscopic and macroscopic studies in neuroscience research requires monitoring of neuronal population dynamics and quantifying specific molecules or genes from the brain of identical animals. To this end, by minimizing the size and weight of an electrode array, we developed a method that records local field potential signals of multiple brain regions from one side of the hemisphere in a freely moving rodent. At the same time, extracellular cerebrospinal fluid for biochemical assays or a small part of brain tissue samples for gene expression assays are collected from the other side of the hemisphere. This method allows ongoing stable recordings and sample collections for at least two months. The methodological concept is applicable to a wide range of biological reactions at various spatiotemporal scales, allowing us to integrate an idea of physiolomics into existing omics analyses, leading to a new combination of multi-omics approaches.

Original languageEnglish
Pages (from-to)346-351
Number of pages6
JournalJournal of Pharmacological Sciences
Volume139
Issue number4
DOIs
Publication statusPublished - 2019 Apr

Keywords

  • Brain
  • Genes
  • Local field potential
  • Microdialysis
  • Neuromodulator

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