TY - JOUR
T1 - Comparison of blood profiles of γ-Oryzanol and ferulic acid in rats after oral intake of γ-Oryzanol
AU - Kokumai, Takumi
AU - Ito, Junya
AU - Kobayashi, Eri
AU - Shimizu, Naoki
AU - Hashimoto, Hiroyuki
AU - Eitsuka, Takahiro
AU - Miyazawa, Teruo
AU - Nakagawa, Kiyotaka
N1 - Funding Information:
Funding: This research was funded in part by JSPS KAKENHI [grant number 17K19217 and 19K15783] and the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety”.
Funding Information:
This research was funded in part by JSPS KAKENHI [grant number 17K19217 and 19K15783] and the JSPS Core-to-Core Program (Advanced Research Networks) entitled “Establishment of international agricultural immunology research-core for a quantum improvement in food safety”. Acknowledgments: We thank Hiroaki Murase, Glovia Co., Ltd., and Isabella Supardi Parida (Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University) for technical assistance in this study.
Publisher Copyright:
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/5
Y1 - 2019/5
N2 - γ-Oryzanol (OZ), a bioactive phytochemical abundant in cereals such as rice, has been reported to be mainly hydrolyzed to ferulic acid (FA) in the body. Meanwhile, in our previous study, we revealed that a part of OZ is absorbed into the body and exists in its intact form. However, the comprehensive absorption profile of OZ and its metabolites (e.g., FA) after OZ intake has not been fully elucidated yet. Therefore, in this study, we measured the concentrations of OZ, FA, and FA conjugates (i.e., FA sulfate and glucuronide) in the blood of rats with the use of HPLC-MS/MS after a single oral administration of 300 µmol/kg body weight of rice bran OZ (RBOZ). As a result, intact OZ along with FA and FA conjugates existed in the blood, which implied that these constituents may all contribute to the physiological effects under OZ intake. Additionally, when an equimolar amount of FA (300 µmol/kg body weight) was administered, it was found that the absorption profile of FA was significantly different from that when RBOZ was administered.
AB - γ-Oryzanol (OZ), a bioactive phytochemical abundant in cereals such as rice, has been reported to be mainly hydrolyzed to ferulic acid (FA) in the body. Meanwhile, in our previous study, we revealed that a part of OZ is absorbed into the body and exists in its intact form. However, the comprehensive absorption profile of OZ and its metabolites (e.g., FA) after OZ intake has not been fully elucidated yet. Therefore, in this study, we measured the concentrations of OZ, FA, and FA conjugates (i.e., FA sulfate and glucuronide) in the blood of rats with the use of HPLC-MS/MS after a single oral administration of 300 µmol/kg body weight of rice bran OZ (RBOZ). As a result, intact OZ along with FA and FA conjugates existed in the blood, which implied that these constituents may all contribute to the physiological effects under OZ intake. Additionally, when an equimolar amount of FA (300 µmol/kg body weight) was administered, it was found that the absorption profile of FA was significantly different from that when RBOZ was administered.
KW - Absorption and metabolism
KW - Ferulic acid
KW - HPLC-MS/MS
KW - Rice bran
KW - γ-oryzanol
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U2 - 10.3390/nu11051174
DO - 10.3390/nu11051174
M3 - Article
C2 - 31130653
AN - SCOPUS:85066930436
SN - 2072-6643
VL - 11
JO - Nutrients
JF - Nutrients
IS - 5
M1 - 1174
ER -