TY - JOUR
T1 - Comparison of OspA serotypes for Borrelia burgdorferi sensu lato from Japan, Europe and North America
AU - Masuzawa, Toshiyuki
AU - Wilske, Bettina
AU - Komikado, Tetsuro
AU - Suzuki, Hiroyuki
AU - Kawabata, Hiroki
AU - Sato, Nanao
AU - Muramatsu, Koichi
AU - Sato, Nobutake
AU - Isogai, Emiko
AU - Isogai, Hiroshi
AU - Johnson, Russell C.
AU - Yanagihara, Yasutake
PY - 1996
Y1 - 1996
N2 - Sixty-one Borrelia burgdorferi sensu lato strains from various sources (ticks, human, and wild animals) in Japan and two strains from ticks in Far Eastern Russia were classified on the basis of reactivity with 16 monoclonal antibodies (mAb) to outer surface protein A (OspA) and by DNA-DNA hybridization assay. Eleven OspA serotypes (J1 to J11) were recognized among the Japanese and the Far East Russian isolates (serotypes J1 to J9 were identified as B. garinii, serotype J10 was identified as B. afzelii, and serotype J11 corresponded to B. japonica), whereas 7 OspA serotypes for North American and European isolates previously reported (Bettina Wilske et al, J. Clin. Microbiol. 31:340-350, 1993) were not observed except for OspA serotype 2 which showed identical reactivity with OspA serotype J10. This finding provides helpful information for understanding the geographical distribution of Lyme disease borrelia and the development of vaccine and diagnostic tests. In conclusion: 1. B. burgdorferi sensu stricto has not been observed in Japan, 2. Japanese B. afzelii isolates are closely related to those from Europe, 3. B. garinii isolates from Japan are highly heterogeneous and apparently different from European B. garinii isolates.
AB - Sixty-one Borrelia burgdorferi sensu lato strains from various sources (ticks, human, and wild animals) in Japan and two strains from ticks in Far Eastern Russia were classified on the basis of reactivity with 16 monoclonal antibodies (mAb) to outer surface protein A (OspA) and by DNA-DNA hybridization assay. Eleven OspA serotypes (J1 to J11) were recognized among the Japanese and the Far East Russian isolates (serotypes J1 to J9 were identified as B. garinii, serotype J10 was identified as B. afzelii, and serotype J11 corresponded to B. japonica), whereas 7 OspA serotypes for North American and European isolates previously reported (Bettina Wilske et al, J. Clin. Microbiol. 31:340-350, 1993) were not observed except for OspA serotype 2 which showed identical reactivity with OspA serotype J10. This finding provides helpful information for understanding the geographical distribution of Lyme disease borrelia and the development of vaccine and diagnostic tests. In conclusion: 1. B. burgdorferi sensu stricto has not been observed in Japan, 2. Japanese B. afzelii isolates are closely related to those from Europe, 3. B. garinii isolates from Japan are highly heterogeneous and apparently different from European B. garinii isolates.
KW - Borrelia burgdorferi
KW - Genospecies
KW - Lyme disease
KW - OspA
UR - http://www.scopus.com/inward/record.url?scp=9444286387&partnerID=8YFLogxK
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U2 - 10.1111/j.1348-0421.1996.tb01106.x
DO - 10.1111/j.1348-0421.1996.tb01106.x
M3 - Article
C2 - 8887347
AN - SCOPUS:9444286387
SN - 0385-5600
VL - 40
SP - 539
EP - 545
JO - Microbiology and Immunology
JF - Microbiology and Immunology
IS - 8
ER -
