TY - JOUR
T1 - Comparison of the multiple agglutinins of the acorn barnacle, megabalanus rosa
AU - Muramoto, Koji
AU - Ogata, Kyoko
AU - Kamiya, Hisao
N1 - Funding Information:
Acknowledgments. We are grateful to Dr. H. Meguro, Tohoku University, for the CD spectra and Dr. H. Kawauchi, Kitasato University, for the amino acid analyses. The technical assistance of S. Sunahara is appreciated. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1985/1
Y1 - 1985/1
N2 - We investigated the subunit structures of the three agglutinins (BRA-1, BRA-2, and BRA-3) isolated from the coelomic fluid of the acorn barnacle, Megabalanus rosa, by high-speed gel filtration, gel electrophoresis, CD spectra, and peptide mapping. BRA-2 (Mr 140,000) and BRA-3 (Mr 64,000) were glycoproteins composed of subunits having two identical basic units cross-linked by disulfide bonds. The basic units of BRA-2 and BRA-3 had molecular weights of 22,000 and 18,000, respectively. They did not have a common structure. BRA-1 (Mr 330,000) was composed of subunits homologous to that of BRA-2. The binding properties of these agglutinins against rabbit erythrocyte ghosts were also investigated after labeling the agglutinins with fluorescein isothiocyanate.
AB - We investigated the subunit structures of the three agglutinins (BRA-1, BRA-2, and BRA-3) isolated from the coelomic fluid of the acorn barnacle, Megabalanus rosa, by high-speed gel filtration, gel electrophoresis, CD spectra, and peptide mapping. BRA-2 (Mr 140,000) and BRA-3 (Mr 64,000) were glycoproteins composed of subunits having two identical basic units cross-linked by disulfide bonds. The basic units of BRA-2 and BRA-3 had molecular weights of 22,000 and 18,000, respectively. They did not have a common structure. BRA-1 (Mr 330,000) was composed of subunits homologous to that of BRA-2. The binding properties of these agglutinins against rabbit erythrocyte ghosts were also investigated after labeling the agglutinins with fluorescein isothiocyanate.
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U2 - 10.1080/00021369.1985.10866675
DO - 10.1080/00021369.1985.10866675
M3 - Article
AN - SCOPUS:0002856565
SN - 0916-8451
VL - 49
SP - 85
EP - 93
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 1
ER -