TY - JOUR
T1 - Comprehensive analysis of FGF and FGFR expression in skin
T2 - FGF18 is highly expressed in hair follicles and capable of inducing anagen from telogen stage hair follicles
AU - Kawano, Mitsuko
AU - Komi-Kuramochi, Akiko
AU - Asada, Masahiro
AU - Suzuki, Masashi
AU - Oki, Junko
AU - Jiang, Ju
AU - Imamura, Toru
N1 - Funding Information:
We thank Prof. Tadashi Baba at the University of Tsukuba for his support of the graduate study of Mitsuko Kawano, and Prof. Akira Suyama at Tokyo University and Olympus Optical Co. Ltd for primer design software using a novel algorithm. We also thank Dr Kevin J. McElwee at the University of British Columbia for his helpful suggestion regarding our hair growth assay. Akiko Komi-Kuramochi was supported in part by a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2005/5
Y1 - 2005/5
N2 - We quantified the mRNA expression of all 22 fibroblast growth factor family members (FGF) and their four receptors (FGFR) in adult mouse full-thickness skin at various stages of the hair growth cycle. We found that in addition to mRNA encoding FGF previously identified in skin (FGF1, 2, 5, 7, 10, 13, and 22), FGF18 mRNA was also strongly expressed. Expression of these FGF varied throughout hair growth cycle: mRNA expression of FGF18 and 13 peaked at telogen; FGF7 and 10 at anagen V; and FGF5 and 22 at anagen VI. In situ hybridization revealed that FGF18 mRNA is mainly expressed in the anagen inner root sheath and telogen bulge of hair follicles. In culture, FGF18 stimulated DNA synthesis in human dermal fibroblasts, dermal papilla cells, epidermal keratinocytes and vascular endothelial cells. When FGF18 was administered subcutaneously to mice in a uniform telogen state, anagen hair growth was observed. Our findings suggest that FGF18 is important for the regulation of hair growth and the maintenance of skin in adult mice.
AB - We quantified the mRNA expression of all 22 fibroblast growth factor family members (FGF) and their four receptors (FGFR) in adult mouse full-thickness skin at various stages of the hair growth cycle. We found that in addition to mRNA encoding FGF previously identified in skin (FGF1, 2, 5, 7, 10, 13, and 22), FGF18 mRNA was also strongly expressed. Expression of these FGF varied throughout hair growth cycle: mRNA expression of FGF18 and 13 peaked at telogen; FGF7 and 10 at anagen V; and FGF5 and 22 at anagen VI. In situ hybridization revealed that FGF18 mRNA is mainly expressed in the anagen inner root sheath and telogen bulge of hair follicles. In culture, FGF18 stimulated DNA synthesis in human dermal fibroblasts, dermal papilla cells, epidermal keratinocytes and vascular endothelial cells. When FGF18 was administered subcutaneously to mice in a uniform telogen state, anagen hair growth was observed. Our findings suggest that FGF18 is important for the regulation of hair growth and the maintenance of skin in adult mice.
KW - Dermis
KW - Fibroblast growth factor
KW - Hair growth
KW - Receptor
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U2 - 10.1111/j.0022-202X.2005.23693.x
DO - 10.1111/j.0022-202X.2005.23693.x
M3 - Article
C2 - 15854025
AN - SCOPUS:18244405300
SN - 0022-202X
VL - 124
SP - 877
EP - 885
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
IS - 5
ER -