Comprehensive analysis of serum microRNAs in autoimmune pancreatitis

Shin Hamada, Atsushi Masamune, Atsushi Kanno, Tooru Shimosegawa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Background/Aims: Autoimmune pancreatitis (AIP) is a rare disease that has recently emerged as a unique type of pancreatitis with a presumed autoimmune etiology. MicroRNA (miRNA) is a small non-coding RNA that targets multiple mRNAs. miRNAs might exist in serum in a stabilized form, suggesting its potential application as a biomarker. We here examined the miRNA expression profile in the serum of patients with AIP. Methods: miRNAs were prepared from serum samples of patients with various pancreatic diseases (AIP (n = 3, each before and after the steroid therapy), chronic pancreatitis (n = 5), pancreatic cancer (n = 5)) or healthy controls (n = 5). A human miRNA Oligo chip containing approximately 2,000 miRNAs was used to identify differentially expressed miRNAs. Ingenuity Pathway Analysis (IPA) was used for the integrated analysis of altered miRNAs. Results: Microarray analysis identified miRNAs highly expressed in the serum of patients with AIP: 13 miRNAs vs. CP, 204 miRNAs vs. pancreatic cancer, and 19 miRNAs vs. healthy controls. miR-150-5p was commonly upregulated in AIP compared to the other samples. IPA revealed the most biological processes affected by the steroid therapy including cellular development, cellular growth, and cell movement. Conclusion: Our results identified that miRNAs were differentially expressed in the serum of AIP patients.

Original languageEnglish
Pages (from-to)263-271
Number of pages9
Issue number4
Publication statusPublished - 2015 Jun 15


  • Biomarker
  • IgG4-related disease
  • Ingenuity pathway analysis
  • Microarray
  • Pancreatic cancer
  • Pancreatitis


Dive into the research topics of 'Comprehensive analysis of serum microRNAs in autoimmune pancreatitis'. Together they form a unique fingerprint.

Cite this