TY - JOUR
T1 - Congenic method in the chick limb buds by electroporation
AU - Suzuki, Takayuki
AU - Ogura, Toshihiko
PY - 2008/8
Y1 - 2008/8
N2 - Electroporation is a powerful tool with which to study limb development. Limb development, however, remains an intricate series of events, requiring the precise dissection of developmental processes using relevant transgenes. In this review, we describe the anatomy of the limb field as the basis of targeted electroporation, and specific expression vectors are discussed. We share a useful protocol for electroporation of chick limb buds, and the expression pattern of enhanced green fluorescent protein in the limb buds is used to demonstrate relevant embryonic patterning. Finally, useful trouble-shooting techniques are described.
AB - Electroporation is a powerful tool with which to study limb development. Limb development, however, remains an intricate series of events, requiring the precise dissection of developmental processes using relevant transgenes. In this review, we describe the anatomy of the limb field as the basis of targeted electroporation, and specific expression vectors are discussed. We share a useful protocol for electroporation of chick limb buds, and the expression pattern of enhanced green fluorescent protein in the limb buds is used to demonstrate relevant embryonic patterning. Finally, useful trouble-shooting techniques are described.
KW - Chick embryo
KW - Electroporation
KW - Limb buds
KW - Limb field
UR - http://www.scopus.com/inward/record.url?scp=50049084142&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50049084142&partnerID=8YFLogxK
U2 - 10.1111/j.1440-169X.2008.01054.x
DO - 10.1111/j.1440-169X.2008.01054.x
M3 - Review article
C2 - 18638168
AN - SCOPUS:50049084142
SN - 0012-1592
VL - 50
SP - 459
EP - 465
JO - Development Growth and Differentiation
JF - Development Growth and Differentiation
IS - 6
ER -