TY - JOUR
T1 - Constitutive and Interleukin-1 (IL-1)-Inducible Factors Interact with the IL-1-Responsive Element in the IL-6 Gene
AU - Isshiki, Hiroshi
AU - Akira, Shizuo
AU - Tanabe, Osamu
AU - Nakajima, Toshihiro
AU - Shimamoto, Takuya
AU - Hirano, Toshio
AU - Kishimoto, Tadamitsu
PY - 1990/6
Y1 - 1990/6
N2 - The interleukin-6 (IL-6) promoter is rapidly and transiently activated with other cytokines, including IL-1, tumor necrosis factor, and platelet-derived growth factor, as well as phorbol esters and agents that increase intracellular cyclic AMP. In this study, we have investigated cis-acting regulatory elements and trans-acting factors responsible for IL-1-induced IL-6 gene expression. Studies on the 5′ deletion mutants of the human IL-6 gene suggested that the IL-1-responsive element was mapped within the IL-6 promoter region (-180 to -123) which was homologous to the c-fos serum-responsive enhancer element. Gel retardation assay identified two types of nuclear factors that bound to this region, one constitutive and the other inducible. These two factors recognized a 14-base-pair (bp) palindromic sequence, ACATTGCACAATCT. Furthermore, three copies of this 14-bp palindrome conferred IL-1 responsiveness to the basal enhancerless IL-6 promoter, indicating that a 14-bp-dyad symmetry sequence was an IL-1-responsive element in the IL-6 gene.
AB - The interleukin-6 (IL-6) promoter is rapidly and transiently activated with other cytokines, including IL-1, tumor necrosis factor, and platelet-derived growth factor, as well as phorbol esters and agents that increase intracellular cyclic AMP. In this study, we have investigated cis-acting regulatory elements and trans-acting factors responsible for IL-1-induced IL-6 gene expression. Studies on the 5′ deletion mutants of the human IL-6 gene suggested that the IL-1-responsive element was mapped within the IL-6 promoter region (-180 to -123) which was homologous to the c-fos serum-responsive enhancer element. Gel retardation assay identified two types of nuclear factors that bound to this region, one constitutive and the other inducible. These two factors recognized a 14-base-pair (bp) palindromic sequence, ACATTGCACAATCT. Furthermore, three copies of this 14-bp palindrome conferred IL-1 responsiveness to the basal enhancerless IL-6 promoter, indicating that a 14-bp-dyad symmetry sequence was an IL-1-responsive element in the IL-6 gene.
UR - http://www.scopus.com/inward/record.url?scp=0025370224&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025370224&partnerID=8YFLogxK
U2 - 10.1128/MCB.10.6.2757
DO - 10.1128/MCB.10.6.2757
M3 - Article
C2 - 2111442
AN - SCOPUS:0025370224
SN - 0270-7306
VL - 10
SP - 2757
EP - 2764
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 6
ER -