Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants

Arata Suenaga; Kanami Moriya; Yutaka Sonoda; Akira Ikeda; Nicolaus Von Wirén; Toshihiko Hayakawa; Junji Yamaguchi; Tomoyuki Yamaya

doi:10.1093/pcp/pcg017

Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants

Arata Suenaga, Kanami Moriya, Yutaka Sonoda, Akira Ikeda, Nicolaus Von Wirén, Toshihiko Hayakawa, Junji Yamaguchi, Tomoyuki Yamaya

AGR - Agricultural Chemistry

Research output: Contribution to journal › Article › peer-review

151 Citations (Scopus)

Abstract

To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammonium-uptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.

Original language	English
Pages (from-to)	206-211
Number of pages	6
Journal	Plant and Cell Physiology
Volume	44
Issue number	2
DOIs	https://doi.org/10.1093/pcp/pcg017
Publication status	Published - 2003 Feb 1

Keywords

Ammonium transporter
AMT2
Antibody
Functional expression
Rice

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title = "Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants",

abstract = "To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammonium-uptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.",

keywords = "Ammonium transporter, AMT2, Antibody, Functional expression, Rice",

author = "Arata Suenaga and Kanami Moriya and Yutaka Sonoda and Akira Ikeda and {Von Wir{\'e}n}, Nicolaus and Toshihiko Hayakawa and Junji Yamaguchi and Tomoyuki Yamaya",

note = "Funding Information: This work was supported in part by a program of CREST of JST (Japan Science Technology) and in part by Grant-in-Aid from the Ministry of Education, Science, Culture, Technology, and Sport of Japan (Nos. 12460029, 14360035 and 14654160). We thank Dr. T. Sasaki of the Japanese Rice Genome Program for providing us with the expressed sequence tag clones and Dr. Bruno Andre of Universite Libre de Bruxelles for yeast mutants and expression vectors for ammonium transport assay.",

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doi = "10.1093/pcp/pcg017",

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AU - Suenaga, Arata

AU - Moriya, Kanami

AU - Sonoda, Yutaka

AU - Ikeda, Akira

AU - Von Wirén, Nicolaus

AU - Hayakawa, Toshihiko

AU - Yamaguchi, Junji

AU - Yamaya, Tomoyuki

N1 - Funding Information: This work was supported in part by a program of CREST of JST (Japan Science Technology) and in part by Grant-in-Aid from the Ministry of Education, Science, Culture, Technology, and Sport of Japan (Nos. 12460029, 14360035 and 14654160). We thank Dr. T. Sasaki of the Japanese Rice Genome Program for providing us with the expressed sequence tag clones and Dr. Bruno Andre of Universite Libre de Bruxelles for yeast mutants and expression vectors for ammonium transport assay.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammonium-uptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.

AB - To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammonium-uptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.

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KW - Antibody

KW - Functional expression

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Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants

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