Constitutive expression of a novel-type ammonium transporter OsAMT2 in rice plants

Arata Suenaga, Kanami Moriya, Yutaka Sonoda, Akira Ikeda, Nicolaus Von Wirén, Toshihiko Hayakawa, Junji Yamaguchi, Tomoyuki Yamaya

Research output: Contribution to journalArticlepeer-review

151 Citations (Scopus)


To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammonium-uptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.

Original languageEnglish
Pages (from-to)206-211
Number of pages6
JournalPlant and Cell Physiology
Issue number2
Publication statusPublished - 2003 Feb 1


  • Ammonium transporter
  • AMT2
  • Antibody
  • Functional expression
  • Rice


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