TY - JOUR
T1 - Construction of a human B cell line, TKHMY, suitable for production of stable human hybridomas
AU - Kudo, Toshio
AU - Saeki, Hisaaki
AU - Katayose, Yu
AU - Yasui, Akira
PY - 1994/12/28
Y1 - 1994/12/28
N2 - In order to produce stable hybridomas for generation of human monoclonal antibodies (HMAbs), an attempt was made to construct a suitable human parental B cell line with double selection markers, the strategy being to produce HMAbs by fusing a parental B cell line with EBV-transformed B cells producing specific antibodies. The resultant TKHMY cell line, constructed by transfecting the Blasticidin S resistant (bsr) gene into the HAT sensitive human B cell line LTR 228 could be used as a fusion partner for very stable, indicating that IgG monoclonal antibodies to antigens on lung cancer cells by one such hybridoma was very stable, indicating that this TKHMY cell line has practical advantage in the area of antibody production.
AB - In order to produce stable hybridomas for generation of human monoclonal antibodies (HMAbs), an attempt was made to construct a suitable human parental B cell line with double selection markers, the strategy being to produce HMAbs by fusing a parental B cell line with EBV-transformed B cells producing specific antibodies. The resultant TKHMY cell line, constructed by transfecting the Blasticidin S resistant (bsr) gene into the HAT sensitive human B cell line LTR 228 could be used as a fusion partner for very stable, indicating that IgG monoclonal antibodies to antigens on lung cancer cells by one such hybridoma was very stable, indicating that this TKHMY cell line has practical advantage in the area of antibody production.
KW - Blasticidin S
KW - Cell fusion
KW - Hybridoma, human
KW - Parental B cell line
UR - http://www.scopus.com/inward/record.url?scp=0028595640&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028595640&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(94)90138-4
DO - 10.1016/0022-1759(94)90138-4
M3 - Article
C2 - 7822823
AN - SCOPUS:0028595640
SN - 0022-1759
VL - 177
SP - 17
EP - 22
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1-2
ER -
