Construction of a human B cell line, TKHMY, suitable for production of stable human hybridomas

Toshio Kudo, Hisaaki Saeki, Yu Katayose, Akira Yasui

    Research output: Contribution to journalArticlepeer-review

    2 Citations (Scopus)


    In order to produce stable hybridomas for generation of human monoclonal antibodies (HMAbs), an attempt was made to construct a suitable human parental B cell line with double selection markers, the strategy being to produce HMAbs by fusing a parental B cell line with EBV-transformed B cells producing specific antibodies. The resultant TKHMY cell line, constructed by transfecting the Blasticidin S resistant (bsr) gene into the HAT sensitive human B cell line LTR 228 could be used as a fusion partner for very stable, indicating that IgG monoclonal antibodies to antigens on lung cancer cells by one such hybridoma was very stable, indicating that this TKHMY cell line has practical advantage in the area of antibody production.

    Original languageEnglish
    Pages (from-to)17-22
    Number of pages6
    JournalJournal of Immunological Methods
    Issue number1-2
    Publication statusPublished - 1994 Dec 28


    • Blasticidin S
    • Cell fusion
    • Hybridoma, human
    • Parental B cell line

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology


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