TY - JOUR
T1 - Construction of a thiamine pyrophosphate high-producing strain of Aspergillus oryzae by overexpression of three genes involved in thiamine biosynthesis
AU - Tokui, Misato
AU - Kubodera, Takafumi
AU - Gomi, Katsuya
AU - Yamashita, Nobuo
AU - Nishimura, Akira
PY - 2011/4
Y1 - 2011/4
N2 - We have found a gene (thiP) encoding thiamine pyrophosphokinase (TPK) in the Aspergillus oryzae genome. No riboswitch-like region was found in the upstream region of thiP, although it was repressed probably by thiamine pyrophosphate (TPP) as well as thiA and nmtA, which are strictly regulated by TPP-riboswitch sequence. To improve the productivity of TPP in A. oryzae, we constructed the strain in which thiA, nmtA and thiP were overexpressed simultaneously. The resulting strain accumulated intracellular TPP 4-fold higher than did the control strain.
AB - We have found a gene (thiP) encoding thiamine pyrophosphokinase (TPK) in the Aspergillus oryzae genome. No riboswitch-like region was found in the upstream region of thiP, although it was repressed probably by thiamine pyrophosphate (TPP) as well as thiA and nmtA, which are strictly regulated by TPP-riboswitch sequence. To improve the productivity of TPP in A. oryzae, we constructed the strain in which thiA, nmtA and thiP were overexpressed simultaneously. The resulting strain accumulated intracellular TPP 4-fold higher than did the control strain.
KW - Aspergillus oryzae
KW - Thiamine biosynthesis
KW - Thiamine production
KW - Thiamine pyrophosphate
KW - Thiamine pyrophosphokinase
UR - http://www.scopus.com/inward/record.url?scp=79953324324&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953324324&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2010.12.011
DO - 10.1016/j.jbiosc.2010.12.011
M3 - Article
C2 - 21247799
AN - SCOPUS:79953324324
SN - 1389-1723
VL - 111
SP - 388
EP - 390
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 4
ER -