Construction of multilayer thin films of enzymes by means of sugar-lectin interactions

Jun Ichi Anzai, Yuka Kobayashi

Research output: Contribution to journalArticlepeer-review

122 Citations (Scopus)


A layer-by-layer deposition of concanavalin A (Con A) and glycoproteins such glucose oxidase (GOx) and horseradish peroxidase (HRP) afforded multilayer thin films on the surfaces of a quartz slide and a platinum electrode, through biospecific complexation of Con A and sugar residues in the glycoenzymes. Lactate oxidase (LOx), which contains intrinsically no sugar chain, was also built into a multilayer assembly by being modified extrinsically with mannose residues. The enzymes formed monomolecular or sub-monomolecular layers in each layer of the multilayer films. Electrochemical measurements using Con A-enzyme multilayer-modified electrodes revealed that the enzymes are catalytically active in the multilayer films. The Con A-enzyme multilayer films are relatively stable against low concentrations of mannose and urea, although the films destructed gradually in high concentrations of urea solutions (>3 M).

Original languageEnglish
Pages (from-to)2851-2856
Number of pages6
Issue number6
Publication statusPublished - 2000 Mar 21

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


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