Construction of T-Motif-Based DNA Nanostructures through Enzymatic Reactions

Ryo Kageyama, Ibuki Kawamata, Kaori Tanabe, Yuki Suzuki, Shin ichiro M. Nomura, Satoshi Murata

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The most common way to fabricate DNA nanostructures is to mix individually synthesized DNA oligomers in one pot. However, if DNA nanostructures could be produced through enzymatic reactions, they could be applied in various environments, including in vivo. Herein, an enzymatic method developed to construct a DNA nanostructure from a simple motif called a T-motif is reported. A long, repeated structure was replicated from a circular template by rolling circle amplification and then cleaved into T-motif segments by restriction enzymes. These motifs have been successfully assembled into a ladder-like nanostructure without purification or controlled annealing. This approach is widely applicable to constructing a variety of DNA nanostructures through enzymatic reactions.

Original languageEnglish
Pages (from-to)873-876
Number of pages4
Issue number8
Publication statusPublished - 2018 Apr 16


  • DNA
  • enzymes
  • nanotechnology
  • rolling-circle amplification
  • self-assembly


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