TY - JOUR
T1 - Contractile Skeletal Muscle Cells Cultured with a Conducting Soft Wire for Effective, Selective Stimulation
AU - Nagamine, Kuniaki
AU - Sato, Hirotaka
AU - Kai, Hiroyuki
AU - Kaji, Hirokazu
AU - Kanzaki, Makoto
AU - Nishizawa, Matsuhiko
N1 - Funding Information:
This work was partly supported by the Center of Innovation Program (COI) from the Japan Science and Technology Agency, JST, and by a Grant-in-Aid for Scientific Research A (25246016) and Challenging Exploratory Research (K15K13315) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Contractile skeletal muscle cells were cultured so as to wrap around an electrode wire to enable their selective stimulation even when they were co-cultured with other electrically-excitable cells. Since the electrode wire was composed of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and polyurethane (PU), which is soft and highly capacitive (∼10 mF cm-2), non-faradaic electrical stimulation with charge/discharge currents could be applied to the surrounding cells without causing significant damage even for longer periods (more than a week). The advantage of this new culture system was demonstrated in the study of chemotactic interaction of monocytes and skeletal muscle cells via myokines.
AB - Contractile skeletal muscle cells were cultured so as to wrap around an electrode wire to enable their selective stimulation even when they were co-cultured with other electrically-excitable cells. Since the electrode wire was composed of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and polyurethane (PU), which is soft and highly capacitive (∼10 mF cm-2), non-faradaic electrical stimulation with charge/discharge currents could be applied to the surrounding cells without causing significant damage even for longer periods (more than a week). The advantage of this new culture system was demonstrated in the study of chemotactic interaction of monocytes and skeletal muscle cells via myokines.
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U2 - 10.1038/s41598-018-20729-y
DO - 10.1038/s41598-018-20729-y
M3 - Article
C2 - 29396483
AN - SCOPUS:85041617911
SN - 2045-2322
VL - 8
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 2253
ER -