Control of effect on the nucleation rate for hen egg white lysozyme crystals under application of an external ac electric field

Haruhiko Koizumi; S. Uda; K. Fujiwara; J. Nozawa

doi:10.1021/la2010985

Control of effect on the nucleation rate for hen egg white lysozyme crystals under application of an external ac electric field

Haruhiko Koizumi, S. Uda, K. Fujiwara, J. Nozawa

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The effect of an external ac electric field on the nucleation rate of hen egg white lysozyme crystals increased with an increase in the concentration of the precipitant used, which enabled the design of an electric double layer (EDL) formed at the inner surface of the drop in the oil. This is attributed to the thickness of the EDL controlled by the ionic strength of the precipitant used. Control of the EDL formed at the interface between the two phases is important to establishing this novel technique for the crystallization of proteins under the application of an external ac electric field.

Original language	English
Pages (from-to)	8333-8338
Number of pages	6
Journal	Langmuir
Volume	27
Issue number	13
DOIs	https://doi.org/10.1021/la2010985
Publication status	Published - 2011 Jul 5

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

Access to Document

10.1021/la2010985

Cite this

@article{6fc82cb7b145416884a366fca9201339,

title = "Control of effect on the nucleation rate for hen egg white lysozyme crystals under application of an external ac electric field",

abstract = "The effect of an external ac electric field on the nucleation rate of hen egg white lysozyme crystals increased with an increase in the concentration of the precipitant used, which enabled the design of an electric double layer (EDL) formed at the inner surface of the drop in the oil. This is attributed to the thickness of the EDL controlled by the ionic strength of the precipitant used. Control of the EDL formed at the interface between the two phases is important to establishing this novel technique for the crystallization of proteins under the application of an external ac electric field.",

author = "Haruhiko Koizumi and S. Uda and K. Fujiwara and J. Nozawa",

year = "2011",

month = jul,

day = "5",

doi = "10.1021/la2010985",

language = "English",

volume = "27",

pages = "8333--8338",

journal = "Langmuir",

issn = "0743-7463",

publisher = "American Chemical Society",

number = "13",

}

TY - JOUR

T1 - Control of effect on the nucleation rate for hen egg white lysozyme crystals under application of an external ac electric field

AU - Koizumi, Haruhiko

AU - Uda, S.

AU - Fujiwara, K.

AU - Nozawa, J.

PY - 2011/7/5

Y1 - 2011/7/5

N2 - The effect of an external ac electric field on the nucleation rate of hen egg white lysozyme crystals increased with an increase in the concentration of the precipitant used, which enabled the design of an electric double layer (EDL) formed at the inner surface of the drop in the oil. This is attributed to the thickness of the EDL controlled by the ionic strength of the precipitant used. Control of the EDL formed at the interface between the two phases is important to establishing this novel technique for the crystallization of proteins under the application of an external ac electric field.

AB - The effect of an external ac electric field on the nucleation rate of hen egg white lysozyme crystals increased with an increase in the concentration of the precipitant used, which enabled the design of an electric double layer (EDL) formed at the inner surface of the drop in the oil. This is attributed to the thickness of the EDL controlled by the ionic strength of the precipitant used. Control of the EDL formed at the interface between the two phases is important to establishing this novel technique for the crystallization of proteins under the application of an external ac electric field.

UR - http://www.scopus.com/inward/record.url?scp=79959791095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959791095&partnerID=8YFLogxK

U2 - 10.1021/la2010985

DO - 10.1021/la2010985

M3 - Article

C2 - 21651297

AN - SCOPUS:79959791095

SN - 0743-7463

VL - 27

SP - 8333

EP - 8338

JO - Langmuir

JF - Langmuir

IS - 13

ER -

Control of effect on the nucleation rate for hen egg white lysozyme crystals under application of an external ac electric field

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this