TY - JOUR
T1 - Conversion of octacalcium phosphate into hydroxyapatite and bone regeneration
AU - Suzuki, Osamu
AU - Kamakura, Shinji
AU - Anada, Takahisa
PY - 2008
Y1 - 2008
N2 - The present study was designed to investigate the mechanism of in vivo conversion from synthetic octacalcium phosphate (OCP) into hydroxyapatite (HA) at ultrastructural level, where the implanted OCP is enhancing bone regeneration in mouse calvarial bone defect. OCP granules were implanted into the subperiosteal area of the calvaria of 7-week-old BALB/c mice for 3 weeks. Transmission electron microscopy of undecalcified frontal sections, obtained from the acrylic resin-embedded skull specimens showed that the bone crystals in newly formed bone directly bonded to the OCP particles implanted. The morphological characteristic of original plate-like OCP particles was remained unchanged even after the implantation, whereas a number of de novo nano-particles were also directly formed onto the plate-like OCP particles. Some of OCP particles were linked with other OCP particles through these nano-particles. The results suggest that the OCP-apatite conversion, involving the enhanced bone regeneration, advances via topotaxial conversion without changing the original OCP morphology, accompanied by solution-mediated de novo nano-apatite formation, in the vicinity of the implanted OCP particles.
AB - The present study was designed to investigate the mechanism of in vivo conversion from synthetic octacalcium phosphate (OCP) into hydroxyapatite (HA) at ultrastructural level, where the implanted OCP is enhancing bone regeneration in mouse calvarial bone defect. OCP granules were implanted into the subperiosteal area of the calvaria of 7-week-old BALB/c mice for 3 weeks. Transmission electron microscopy of undecalcified frontal sections, obtained from the acrylic resin-embedded skull specimens showed that the bone crystals in newly formed bone directly bonded to the OCP particles implanted. The morphological characteristic of original plate-like OCP particles was remained unchanged even after the implantation, whereas a number of de novo nano-particles were also directly formed onto the plate-like OCP particles. Some of OCP particles were linked with other OCP particles through these nano-particles. The results suggest that the OCP-apatite conversion, involving the enhanced bone regeneration, advances via topotaxial conversion without changing the original OCP morphology, accompanied by solution-mediated de novo nano-apatite formation, in the vicinity of the implanted OCP particles.
KW - Bone regeneration
KW - Hydroxyapatite
KW - Octacalcium phosphate
KW - Topotaxial conversion
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M3 - Article
AN - SCOPUS:38149031693
SN - 1013-9826
VL - 361-363 II
SP - 993
EP - 996
JO - Key Engineering Materials
JF - Key Engineering Materials
ER -