Cooperative DNA probing using a β-cyclodextrin DNA conjugate and a nucleobase-specific fluorescent ligand

Toshihiro Ihara; Asuka Uemura; Akika Futamura; Masamichi Shimizu; Noriyuki Baba; Seiichi Nishizawa; Norio Teramae; Akinori Jyo

doi:10.1021/ja809023n

Cooperative DNA probing using a β-cyclodextrin DNA conjugate and a nucleobase-specific fluorescent ligand

Toshihiro Ihara, Asuka Uemura, Akika Futamura, Masamichi Shimizu, Noriyuki Baba, Seiichi Nishizawa, Norio Teramae, Akinori Jyo

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

72 Citations (Scopus)

Abstract

A single nucleotide polymorphism (SNP) base on the target is displayed at a gap in a ternary duplex carrying A-cyclodextrin-modified DNA. A stable tandem duplex forms regardless of the type of SNP base. A nucleobase-specific ligand is then added to this system. The dansyl moiety in the ligand is expected to form a luminous inclusion complex with nearby A-CyD, only when the ligand recognizes the specific base displayed in the gap.

Original language	English
Pages (from-to)	1386-1387
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	131
Issue number	4
DOIs	https://doi.org/10.1021/ja809023n
Publication status	Published - 2009 Feb 4

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10.1021/ja809023n

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abstract = "A single nucleotide polymorphism (SNP) base on the target is displayed at a gap in a ternary duplex carrying A-cyclodextrin-modified DNA. A stable tandem duplex forms regardless of the type of SNP base. A nucleobase-specific ligand is then added to this system. The dansyl moiety in the ligand is expected to form a luminous inclusion complex with nearby A-CyD, only when the ligand recognizes the specific base displayed in the gap.",

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AU - Ihara, Toshihiro

AU - Uemura, Asuka

AU - Futamura, Akika

AU - Shimizu, Masamichi

AU - Baba, Noriyuki

AU - Nishizawa, Seiichi

AU - Teramae, Norio

AU - Jyo, Akinori

PY - 2009/2/4

Y1 - 2009/2/4

N2 - A single nucleotide polymorphism (SNP) base on the target is displayed at a gap in a ternary duplex carrying A-cyclodextrin-modified DNA. A stable tandem duplex forms regardless of the type of SNP base. A nucleobase-specific ligand is then added to this system. The dansyl moiety in the ligand is expected to form a luminous inclusion complex with nearby A-CyD, only when the ligand recognizes the specific base displayed in the gap.

AB - A single nucleotide polymorphism (SNP) base on the target is displayed at a gap in a ternary duplex carrying A-cyclodextrin-modified DNA. A stable tandem duplex forms regardless of the type of SNP base. A nucleobase-specific ligand is then added to this system. The dansyl moiety in the ligand is expected to form a luminous inclusion complex with nearby A-CyD, only when the ligand recognizes the specific base displayed in the gap.

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Cooperative DNA probing using a β-cyclodextrin DNA conjugate and a nucleobase-specific fluorescent ligand

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