Covalent protein labeling with a lanthanide complex and its application to photoluminescence lifetime-based multicolor bioimaging

Shin Mizukami; Taku Yamamoto; Akimasa Yoshimura; Shuji Watanabe; Kazuya Kikuchi

doi:10.1002/anie.201103775

Covalent protein labeling with a lanthanide complex and its application to photoluminescence lifetime-based multicolor bioimaging

Shin Mizukami, Taku Yamamoto, Akimasa Yoshimura, Shuji Watanabe, Kazuya Kikuchi

Research output: Contribution to journal › Article › peer-review

56 Citations (Scopus)

Abstract

Better late â∥ï Lanthanide-based time-resolved luminescence imaging of proteins is a promising technology. A novel luminescent lanthanide probe (see picture, TPA-Eu) for mutant β-lactamase-based protein labeling system (BL-tag) is used to selectively image cell-surface proteins (POIs) in the presence of autofluorescent background signals, thus enabling imaging with serum.

Original language	English
Pages (from-to)	8750-8752
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	37
DOIs	https://doi.org/10.1002/anie.201103775
Publication status	Published - 2011 Sept 5
Externally published	Yes

Keywords

imaging agents
labeling probes
proteins
time-resolved luminescence

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201103775

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AU - Yoshimura, Akimasa

AU - Watanabe, Shuji

AU - Kikuchi, Kazuya

PY - 2011/9/5

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KW - proteins

KW - time-resolved luminescence

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Covalent protein labeling with a lanthanide complex and its application to photoluminescence lifetime-based multicolor bioimaging

Abstract

Keywords

ASJC Scopus subject areas

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