Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

Seiji Sakamoto; Mika Terauchi; Anna Hugo; Tanner Kim; Yasuyuki Araki; Takehiko Wada

doi:10.1039/c3cc43389g

Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

Seiji Sakamoto, Mika Terauchi, Anna Hugo, Tanner Kim, Yasuyuki Araki, Takehiko Wada

IMRAM - Division of Organic- and Biomaterials Research

Tohoku University

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A genetically encodable caspase-3 sensing system has been created using self-assembling split-GFP, in which a C-terminal fragment is “covalently” cyclized via a caspase-3 substrate sequence mediated by split-intein. The specific cleavage of the cyclic C-terminal fragment by caspase-3 induces the GFP reassembly and fluorescence recovery.

Original language	English
Pages (from-to)	10323-10325
Number of pages	3
Journal	Chemical Communications
Volume	49
Issue number	87
DOIs	https://doi.org/10.1039/c3cc43389g
Publication status	Published - 2013 Oct 8

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abstract = "A genetically encodable caspase-3 sensing system has been created using self-assembling split-GFP, in which a C-terminal fragment is “covalently” cyclized via a caspase-3 substrate sequence mediated by split-intein. The specific cleavage of the cyclic C-terminal fragment by caspase-3 induces the GFP reassembly and fluorescence recovery.",

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AU - Araki, Yasuyuki

AU - Wada, Takehiko

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Creation of a caspase-3 sensing system using a combination of split-GFP and split-intein

Abstract

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