Cross-fertilization for enhancing tocotrienol biosynthesis in rice plants and qtl analysis of their F2 progenies

Phumon Sookwong; Kazumasa Murata; Kiyotaka Nakagawa; Akira Shibata; Toshiyuki Kimura; Masayuki Yamaguchi; Yoichiro Kojima; Teruo Miyazawa

doi:10.1021/jf900394t

Cross-fertilization for enhancing tocotrienol biosynthesis in rice plants and qtl analysis of their F₂ progenies

Phumon Sookwong, Kazumasa Murata, Kiyotaka Nakagawa, Akira Shibata, Toshiyuki Kimura, Masayuki Yamaguchi, Yoichiro Kojima, Teruo Miyazawa

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

As rice bran tocotrienol (T3) has been known to have a wide range of physiological functions (e.g., antiangiogenesls), we aimed at developing a T3-rlch rice variety for nutraceutical purposes. T3 content In more than 250 kinds of rice bran samples were Investigated, and Milyang23 was found as the best variety rich in T3. The variety was therefore chosen for cross-fertilization with Koshihikari. Among obtained F₂ progenies, some of them became improved in T3 content (up to 2-fold of reference Koshihikari). QTL analysis of the F₂ progenies revealed five putative loci corresponding to T3 biosynthesis, in which the main loci were located near a marker RM3827 on chromosome 6. The results show that cross-breeding is effective in improving rice bran T3 and provides more genetic understanding on T3 biosynthesis in rice plants.

Original language	English
Pages (from-to)	4620-4625
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	57
Issue number	11
DOIs	https://doi.org/10.1021/jf900394t
Publication status	Published - 2009 Jun 10

Keywords

Oryza sativa L.
Quantitative trait locus
Tocopherol
Tocotrienol

ASJC Scopus subject areas

Chemistry(all)
Agricultural and Biological Sciences(all)

Access to Document

10.1021/jf900394t

Cite this

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title = "Cross-fertilization for enhancing tocotrienol biosynthesis in rice plants and qtl analysis of their F2 progenies",

abstract = "As rice bran tocotrienol (T3) has been known to have a wide range of physiological functions (e.g., antiangiogenesls), we aimed at developing a T3-rlch rice variety for nutraceutical purposes. T3 content In more than 250 kinds of rice bran samples were Investigated, and Milyang23 was found as the best variety rich in T3. The variety was therefore chosen for cross-fertilization with Koshihikari. Among obtained F2 progenies, some of them became improved in T3 content (up to 2-fold of reference Koshihikari). QTL analysis of the F2 progenies revealed five putative loci corresponding to T3 biosynthesis, in which the main loci were located near a marker RM3827 on chromosome 6. The results show that cross-breeding is effective in improving rice bran T3 and provides more genetic understanding on T3 biosynthesis in rice plants.",

keywords = "Oryza sativa L., Quantitative trait locus, Tocopherol, Tocotrienol",

author = "Phumon Sookwong and Kazumasa Murata and Kiyotaka Nakagawa and Akira Shibata and Toshiyuki Kimura and Masayuki Yamaguchi and Yoichiro Kojima and Teruo Miyazawa",

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AU - Sookwong, Phumon

AU - Murata, Kazumasa

AU - Nakagawa, Kiyotaka

AU - Shibata, Akira

AU - Kimura, Toshiyuki

AU - Yamaguchi, Masayuki

AU - Kojima, Yoichiro

AU - Miyazawa, Teruo

PY - 2009/6/10

Y1 - 2009/6/10

N2 - As rice bran tocotrienol (T3) has been known to have a wide range of physiological functions (e.g., antiangiogenesls), we aimed at developing a T3-rlch rice variety for nutraceutical purposes. T3 content In more than 250 kinds of rice bran samples were Investigated, and Milyang23 was found as the best variety rich in T3. The variety was therefore chosen for cross-fertilization with Koshihikari. Among obtained F2 progenies, some of them became improved in T3 content (up to 2-fold of reference Koshihikari). QTL analysis of the F2 progenies revealed five putative loci corresponding to T3 biosynthesis, in which the main loci were located near a marker RM3827 on chromosome 6. The results show that cross-breeding is effective in improving rice bran T3 and provides more genetic understanding on T3 biosynthesis in rice plants.

AB - As rice bran tocotrienol (T3) has been known to have a wide range of physiological functions (e.g., antiangiogenesls), we aimed at developing a T3-rlch rice variety for nutraceutical purposes. T3 content In more than 250 kinds of rice bran samples were Investigated, and Milyang23 was found as the best variety rich in T3. The variety was therefore chosen for cross-fertilization with Koshihikari. Among obtained F2 progenies, some of them became improved in T3 content (up to 2-fold of reference Koshihikari). QTL analysis of the F2 progenies revealed five putative loci corresponding to T3 biosynthesis, in which the main loci were located near a marker RM3827 on chromosome 6. The results show that cross-breeding is effective in improving rice bran T3 and provides more genetic understanding on T3 biosynthesis in rice plants.

KW - Oryza sativa L.

KW - Quantitative trait locus

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KW - Tocotrienol

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