TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of- Hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26
AU - Okai, Masahiko
AU - Kubota, Keiko
AU - Fukuda, Masao
AU - Nagata, Yuji
AU - Nagata, Koji
AU - Tanokura, Masaru
PY - 2009
Y1 - 2009
N2 - LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from-hexachlorocyclohexane (-HCH) to 1,3,4,6-tetrachloro-1, 4-cyclohexadiene (1,4-TCDN) via-pentachlorocyclohexene (-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P41 or P43, with unit-cell parameters a = b = 68.9, c = 101.9 Å, and diffracted X-rays to 2.25 Å resolution. The crystal contained three molecules in the asymmetric unit.
AB - LinA from Sphingobium japonicum UT26 catalyzes two steps of dehydrochlorination from-hexachlorocyclohexane (-HCH) to 1,3,4,6-tetrachloro-1, 4-cyclohexadiene (1,4-TCDN) via-pentachlorocyclohexene (-PCCH). LinA was crystallized by the sitting-drop vapour-diffusion method using PEG 3350 as the precipitant. The crystals belonged to space group P41 or P43, with unit-cell parameters a = b = 68.9, c = 101.9 Å, and diffracted X-rays to 2.25 Å resolution. The crystal contained three molecules in the asymmetric unit.
KW - -hexachlorocyclohexane dehydrochlorinase
KW - LinA
KW - Sphingobium japonicum UT26
UR - http://www.scopus.com/inward/record.url?scp=68649105232&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68649105232&partnerID=8YFLogxK
U2 - 10.1107/S1744309109026645
DO - 10.1107/S1744309109026645
M3 - Article
C2 - 19652349
AN - SCOPUS:68649105232
SN - 1744-3091
VL - 65
SP - 822
EP - 824
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 8
ER -