Crystallization and preliminary X-ray analysis of β-amylase from Bacillus polymyxa

Takashi Yamane, Hiroshi Tasaki, Fusako Matsumoto, Atsuo Suzuki, Nobuyuki Uozumi, Tamaichi Ashida

Research output: Contribution to journalArticlepeer-review


A truncated β-amylase (E.C. from Bacillus polymyxa has been crystallized using the hanging-drop vapour-diffusion method at 277 K. The crystals belong to the orthorhombic space group P212121 with cell dimensions a = 64.6, b = 141.9, c = 155.1 Å and diffract to 2.5 Å resolution. The asymmetric unit containing three protein molecules was derived from an electron-density map calculated at 4 Å resolution using MIR phases. This gives a V(m) value of 2.36 Å3 Da-1.

Original languageEnglish
Pages (from-to)898-900
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Issue number4
Publication statusPublished - 1999 Apr
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology


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