Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters

Margherita Festa, Velia Minicozzi, Anna Boccaccio, Laura Lagostena, Antonella Gradogna, Tianwen Qi, Alex Costa, Nina Larisch, Shin Hamamoto, Emanuela Pedrazzini, Stefan Milenkovic, Joachim Scholz-Starke, Matteo Ceccarelli, Alessandro Vitale, Petra Dietrich, Nobuyuki Uozumi, Franco Gambale, Armando Carpaneto

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


A distinct set of channels and transporters regulates the ion fluxes across the lysosomal membrane. Malfunctioning of these transport proteins and the resulting ionic imbalance is involved in various human diseases, such as lysosomal storage disorders, cancer, as well as metabolic and neurodegenerative diseases. As a consequence, these proteins have stimulated strong interest for their suitability as possible drug targets. A detailed functional characterization of many lysosomal channels and transporters is lacking, mainly due to technical difficulties in applying the standard patch-clamp technique to these small intracellular compartments. In this review, we focus on current methods used to unravel the functional properties of lysosomal ion channels and transporters, stressing their advantages and disadvantages and evaluating their fields of applicability.

Original languageEnglish
Article number921
Issue number6
Publication statusPublished - 2022 Mar 1
Externally publishedYes


  • Ion channels
  • Lysosomes
  • Patch-clamp
  • Plant vacuole
  • Transporters

ASJC Scopus subject areas

  • Medicine(all)


Dive into the research topics of 'Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters'. Together they form a unique fingerprint.

Cite this