Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters

Margherita Festa; Velia Minicozzi; Anna Boccaccio; Laura Lagostena; Antonella Gradogna; Tianwen Qi; Alex Costa; Nina Larisch; Shin Hamamoto; Emanuela Pedrazzini; Stefan Milenkovic; Joachim Scholz-Starke; Matteo Ceccarelli; Alessandro Vitale; Petra Dietrich; Nobuyuki Uozumi; Franco Gambale; Armando Carpaneto

doi:10.3390/cells11060921

Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters

Margherita Festa, Velia Minicozzi, Anna Boccaccio, Laura Lagostena, Antonella Gradogna, Tianwen Qi, Alex Costa, Nina Larisch, Shin Hamamoto, Emanuela Pedrazzini, Stefan Milenkovic, Joachim Scholz-Starke, Matteo Ceccarelli, Alessandro Vitale, Petra Dietrich, Nobuyuki Uozumi, Franco Gambale, Armando Carpaneto

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A distinct set of channels and transporters regulates the ion fluxes across the lysosomal membrane. Malfunctioning of these transport proteins and the resulting ionic imbalance is involved in various human diseases, such as lysosomal storage disorders, cancer, as well as metabolic and neurodegenerative diseases. As a consequence, these proteins have stimulated strong interest for their suitability as possible drug targets. A detailed functional characterization of many lysosomal channels and transporters is lacking, mainly due to technical difficulties in applying the standard patch-clamp technique to these small intracellular compartments. In this review, we focus on current methods used to unravel the functional properties of lysosomal ion channels and transporters, stressing their advantages and disadvantages and evaluating their fields of applicability.

Original language	English
Article number	921
Journal	Cells
Volume	11
Issue number	6
DOIs	https://doi.org/10.3390/cells11060921
Publication status	Published - 2022 Mar 1
Externally published	Yes

Keywords

Ion channels
Lysosomes
Patch-clamp
Plant vacuole
Transporters

ASJC Scopus subject areas

Medicine(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/cells11060921

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Festa, M., Minicozzi, V., Boccaccio, A., Lagostena, L., Gradogna, A., Qi, T., Costa, A., Larisch, N., Hamamoto, S., Pedrazzini, E., Milenkovic, S., Scholz-Starke, J., Ceccarelli, M., Vitale, A., Dietrich, P., Uozumi, N., Gambale, F., & Carpaneto, A. (2022). Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters. Cells, 11(6), [921]. https://doi.org/10.3390/cells11060921

Festa, M, Minicozzi, V, Boccaccio, A, Lagostena, L, Gradogna, A, Qi, T, Costa, A, Larisch, N, Hamamoto, S, Pedrazzini, E, Milenkovic, S, Scholz-Starke, J, Ceccarelli, M, Vitale, A, Dietrich, P, Uozumi, N, Gambale, F & Carpaneto, A 2022, 'Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters', Cells, vol. 11, no. 6, 921. https://doi.org/10.3390/cells11060921

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abstract = "A distinct set of channels and transporters regulates the ion fluxes across the lysosomal membrane. Malfunctioning of these transport proteins and the resulting ionic imbalance is involved in various human diseases, such as lysosomal storage disorders, cancer, as well as metabolic and neurodegenerative diseases. As a consequence, these proteins have stimulated strong interest for their suitability as possible drug targets. A detailed functional characterization of many lysosomal channels and transporters is lacking, mainly due to technical difficulties in applying the standard patch-clamp technique to these small intracellular compartments. In this review, we focus on current methods used to unravel the functional properties of lysosomal ion channels and transporters, stressing their advantages and disadvantages and evaluating their fields of applicability.",

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AU - Festa, Margherita

AU - Minicozzi, Velia

AU - Boccaccio, Anna

AU - Lagostena, Laura

AU - Gradogna, Antonella

AU - Qi, Tianwen

AU - Costa, Alex

AU - Larisch, Nina

AU - Hamamoto, Shin

AU - Pedrazzini, Emanuela

AU - Milenkovic, Stefan

AU - Scholz-Starke, Joachim

AU - Ceccarelli, Matteo

AU - Vitale, Alessandro

AU - Dietrich, Petra

AU - Uozumi, Nobuyuki

AU - Gambale, Franco

AU - Carpaneto, Armando

N1 - Funding Information: Funding: This work was supported by Grants-in-Aid for Scientific Research (20KK0127 and 21K19060) from the Ministry of Education, Culture, Sports, Science and Technology and Moonshot (J200002841) from JST to NU. Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/3/1

Y1 - 2022/3/1

N2 - A distinct set of channels and transporters regulates the ion fluxes across the lysosomal membrane. Malfunctioning of these transport proteins and the resulting ionic imbalance is involved in various human diseases, such as lysosomal storage disorders, cancer, as well as metabolic and neurodegenerative diseases. As a consequence, these proteins have stimulated strong interest for their suitability as possible drug targets. A detailed functional characterization of many lysosomal channels and transporters is lacking, mainly due to technical difficulties in applying the standard patch-clamp technique to these small intracellular compartments. In this review, we focus on current methods used to unravel the functional properties of lysosomal ion channels and transporters, stressing their advantages and disadvantages and evaluating their fields of applicability.

AB - A distinct set of channels and transporters regulates the ion fluxes across the lysosomal membrane. Malfunctioning of these transport proteins and the resulting ionic imbalance is involved in various human diseases, such as lysosomal storage disorders, cancer, as well as metabolic and neurodegenerative diseases. As a consequence, these proteins have stimulated strong interest for their suitability as possible drug targets. A detailed functional characterization of many lysosomal channels and transporters is lacking, mainly due to technical difficulties in applying the standard patch-clamp technique to these small intracellular compartments. In this review, we focus on current methods used to unravel the functional properties of lysosomal ion channels and transporters, stressing their advantages and disadvantages and evaluating their fields of applicability.

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Current Methods to Unravel the Functional Properties of Lysosomal Ion Channels and Transporters

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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