Cyclic ADP-ribose modulates Ca2+ release channels for activation by physiological Ca2+ entry in bullfrog sympathetic neurons

Shao Ying Hua; Takayuki Tokimasa; Shin Takasawa; Yasuhito Furuya; Mitsuo Nohmi; Hiroshi Okamoto; Kenji Kuba

doi:10.1016/0896-6273(94)90315-8

Cyclic ADP-ribose modulates Ca²⁺ release channels for activation by physiological Ca²⁺ entry in bullfrog sympathetic neurons

Shao Ying Hua, Takayuki Tokimasa, Shin Takasawa, Yasuhito Furuya, Mitsuo Nohmi, Hiroshi Okamoto, Kenji Kuba

HOSP - Surgery

Research output: Contribution to journal › Article › peer-review

127 Citations (Scopus)

Abstract

Although Ca²⁺-induced Ca²⁺ release (CICR) via ryanodine receptors has been found to occur in intact neurons, little is known about the physiological processes that regulate it. We studied the effects of cyclic ADP-ribose (cADPR) on CICR in cultured bullfrog sympathetic neurons by fura-2 fluorescence recording and patch-clamp techniques. cADPR applied through a patch pipette augmented action potential- or depolarizing pulse-induced rises in intracellular Ca²⁺ without a change in Ca²⁺ entry initiating the responses, but not in the presence of ryanodine. Likewise, cADPR enhanced a single or oscillatory rise(s) in intracellular Ca²⁺ induced by caffeine. These results strongly suggest that cADPR can be an endogenous modulator of ryanodine receptors in neurons.

Original language	English
Pages (from-to)	1073-1079
Number of pages	7
Journal	Neuron
Volume	12
Issue number	5
DOIs	https://doi.org/10.1016/0896-6273(94)90315-8
Publication status	Published - 1994 May

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title = "Cyclic ADP-ribose modulates Ca2+ release channels for activation by physiological Ca2+ entry in bullfrog sympathetic neurons",

abstract = "Although Ca2+-induced Ca2+ release (CICR) via ryanodine receptors has been found to occur in intact neurons, little is known about the physiological processes that regulate it. We studied the effects of cyclic ADP-ribose (cADPR) on CICR in cultured bullfrog sympathetic neurons by fura-2 fluorescence recording and patch-clamp techniques. cADPR applied through a patch pipette augmented action potential- or depolarizing pulse-induced rises in intracellular Ca2+ without a change in Ca2+ entry initiating the responses, but not in the presence of ryanodine. Likewise, cADPR enhanced a single or oscillatory rise(s) in intracellular Ca2+ induced by caffeine. These results strongly suggest that cADPR can be an endogenous modulator of ryanodine receptors in neurons.",

author = "Hua, {Shao Ying} and Takayuki Tokimasa and Shin Takasawa and Yasuhito Furuya and Mitsuo Nohmi and Hiroshi Okamoto and Kenji Kuba",

note = "Funding Information: We thank Ms. S. Yamakita for excellent secretaria! assistance. This study was supported by a Grant in Aid for Scientific Research (04454139 to K. K.) and a Grant in Aid for Scientific Research on Priority Areas (04267104) from the Japanese Ministry of Education, Science and Culture.",

year = "1994",

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doi = "10.1016/0896-6273(94)90315-8",

language = "English",

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T1 - Cyclic ADP-ribose modulates Ca2+ release channels for activation by physiological Ca2+ entry in bullfrog sympathetic neurons

AU - Hua, Shao Ying

AU - Tokimasa, Takayuki

AU - Takasawa, Shin

AU - Furuya, Yasuhito

AU - Nohmi, Mitsuo

AU - Okamoto, Hiroshi

AU - Kuba, Kenji

N1 - Funding Information: We thank Ms. S. Yamakita for excellent secretaria! assistance. This study was supported by a Grant in Aid for Scientific Research (04454139 to K. K.) and a Grant in Aid for Scientific Research on Priority Areas (04267104) from the Japanese Ministry of Education, Science and Culture.

PY - 1994/5

Y1 - 1994/5

N2 - Although Ca2+-induced Ca2+ release (CICR) via ryanodine receptors has been found to occur in intact neurons, little is known about the physiological processes that regulate it. We studied the effects of cyclic ADP-ribose (cADPR) on CICR in cultured bullfrog sympathetic neurons by fura-2 fluorescence recording and patch-clamp techniques. cADPR applied through a patch pipette augmented action potential- or depolarizing pulse-induced rises in intracellular Ca2+ without a change in Ca2+ entry initiating the responses, but not in the presence of ryanodine. Likewise, cADPR enhanced a single or oscillatory rise(s) in intracellular Ca2+ induced by caffeine. These results strongly suggest that cADPR can be an endogenous modulator of ryanodine receptors in neurons.

AB - Although Ca2+-induced Ca2+ release (CICR) via ryanodine receptors has been found to occur in intact neurons, little is known about the physiological processes that regulate it. We studied the effects of cyclic ADP-ribose (cADPR) on CICR in cultured bullfrog sympathetic neurons by fura-2 fluorescence recording and patch-clamp techniques. cADPR applied through a patch pipette augmented action potential- or depolarizing pulse-induced rises in intracellular Ca2+ without a change in Ca2+ entry initiating the responses, but not in the presence of ryanodine. Likewise, cADPR enhanced a single or oscillatory rise(s) in intracellular Ca2+ induced by caffeine. These results strongly suggest that cADPR can be an endogenous modulator of ryanodine receptors in neurons.

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JO - Neuron

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Cyclic ADP-ribose modulates Ca²⁺ release channels for activation by physiological Ca²⁺ entry in bullfrog sympathetic neurons

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