Cyclic sample pooling using two-dimensional liquid chromatography system enhances coverage in shotgun proteomics

Yusuke Kawashima; Mamoru Satoh; Tatsuya Saito; Takashi Matsui; Fumio Nomura; Hiroyuki Matsumoto; Yoshio Kodera

doi:10.1002/bmc.2864

Cyclic sample pooling using two-dimensional liquid chromatography system enhances coverage in shotgun proteomics

Yusuke Kawashima, Mamoru Satoh, Tatsuya Saito, Takashi Matsui, Fumio Nomura, Hiroyuki Matsumoto, Yoshio Kodera

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

We report a cyclic sample pooling technique devised in two-dimensional liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) shotgun proteomics that renders deeper proteome coverage; we combined low pH reversed-phase (RP) LC in trifluoroacetic acid in the first dimension, followed by cyclic sample pooling of the eluate and low-pH RP-LC in formic acid in the second dimension. The new protocol has a significantly higher resolving power suitable for LC-ESI-MS/MS shotgun proteomics.

Original language	English
Pages (from-to)	691-694
Number of pages	4
Journal	Biomedical Chromatography
Volume	27
Issue number	6
DOIs	https://doi.org/10.1002/bmc.2864
Publication status	Published - 2013 Jun

Keywords

2D-LC-ESI-MS
Cyclic sample pooling
Serum proteins
Shotgun proteomics

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Molecular Biology
Pharmacology
Drug Discovery
Clinical Biochemistry

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10.1002/bmc.2864

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abstract = "We report a cyclic sample pooling technique devised in two-dimensional liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) shotgun proteomics that renders deeper proteome coverage; we combined low pH reversed-phase (RP) LC in trifluoroacetic acid in the first dimension, followed by cyclic sample pooling of the eluate and low-pH RP-LC in formic acid in the second dimension. The new protocol has a significantly higher resolving power suitable for LC-ESI-MS/MS shotgun proteomics.",

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AU - Kawashima, Yusuke

AU - Satoh, Mamoru

AU - Saito, Tatsuya

AU - Matsui, Takashi

AU - Nomura, Fumio

AU - Matsumoto, Hiroyuki

AU - Kodera, Yoshio

PY - 2013/6

Y1 - 2013/6

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AB - We report a cyclic sample pooling technique devised in two-dimensional liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) shotgun proteomics that renders deeper proteome coverage; we combined low pH reversed-phase (RP) LC in trifluoroacetic acid in the first dimension, followed by cyclic sample pooling of the eluate and low-pH RP-LC in formic acid in the second dimension. The new protocol has a significantly higher resolving power suitable for LC-ESI-MS/MS shotgun proteomics.

KW - 2D-LC-ESI-MS

KW - Cyclic sample pooling

KW - Serum proteins

KW - Shotgun proteomics

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Cyclic sample pooling using two-dimensional liquid chromatography system enhances coverage in shotgun proteomics

Abstract

Keywords

ASJC Scopus subject areas

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