Cytotoxicity analysis of staphylococcal bi-component β-pore forming toxins using the CHO cells expressing human lymphocyte receptor CCR5

Zhao Peng; Nao Shibata; Hideaki Tada; Jun Kaneko

doi:10.1080/09168451.2018.1515614

Cytotoxicity analysis of staphylococcal bi-component β-pore forming toxins using the CHO cells expressing human lymphocyte receptor CCR5

Zhao Peng, Nao Shibata, Hideaki Tada, Jun Kaneko

Research output: Contribution to journal › Article › peer-review

Abstract

CCR5-mediated cytotoxicity of staphylococcal bi-component toxins was investigated using human CCR5-expressing CHO cells. Cytotoxicity of rim domain loop-exchange mutants between LukE and Hlg2 indicated that loop-4 of LukE is essential for cytotoxicity in combination with LukD. Interestingly, Hlg2 showed LukF-dependent CCR5-mediated cytotoxicity, suggesting that the F-components of toxins also play a role in the cell-specific cytotoxicity.

Original language	English
Pages (from-to)	2094-2097
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	82
Issue number	12
DOIs	https://doi.org/10.1080/09168451.2018.1515614
Publication status	Published - 2018

Keywords

CCR5
Cytotoxicity
Staphylococcal β-PFTs

ASJC Scopus subject areas

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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10.1080/09168451.2018.1515614

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title = "Cytotoxicity analysis of staphylococcal bi-component β-pore forming toxins using the CHO cells expressing human lymphocyte receptor CCR5",

abstract = "CCR5-mediated cytotoxicity of staphylococcal bi-component toxins was investigated using human CCR5-expressing CHO cells. Cytotoxicity of rim domain loop-exchange mutants between LukE and Hlg2 indicated that loop-4 of LukE is essential for cytotoxicity in combination with LukD. Interestingly, Hlg2 showed LukF-dependent CCR5-mediated cytotoxicity, suggesting that the F-components of toxins also play a role in the cell-specific cytotoxicity.",

keywords = "CCR5, Cytotoxicity, Staphylococcal β-PFTs",

author = "Zhao Peng and Nao Shibata and Hideaki Tada and Jun Kaneko",

note = "Funding Information: This work was supported by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Challenging Exploratory Research under Grant Number [JP16K14897] Publisher Copyright: {\textcopyright} 2018 Japan Society for Bioscience, Biotechnology, and Agrochemistry",

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doi = "10.1080/09168451.2018.1515614",

language = "English",

volume = "82",

pages = "2094--2097",

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T1 - Cytotoxicity analysis of staphylococcal bi-component β-pore forming toxins using the CHO cells expressing human lymphocyte receptor CCR5

AU - Peng, Zhao

AU - Shibata, Nao

AU - Tada, Hideaki

AU - Kaneko, Jun

N1 - Funding Information: This work was supported by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Challenging Exploratory Research under Grant Number [JP16K14897] Publisher Copyright: © 2018 Japan Society for Bioscience, Biotechnology, and Agrochemistry

PY - 2018

Y1 - 2018

N2 - CCR5-mediated cytotoxicity of staphylococcal bi-component toxins was investigated using human CCR5-expressing CHO cells. Cytotoxicity of rim domain loop-exchange mutants between LukE and Hlg2 indicated that loop-4 of LukE is essential for cytotoxicity in combination with LukD. Interestingly, Hlg2 showed LukF-dependent CCR5-mediated cytotoxicity, suggesting that the F-components of toxins also play a role in the cell-specific cytotoxicity.

AB - CCR5-mediated cytotoxicity of staphylococcal bi-component toxins was investigated using human CCR5-expressing CHO cells. Cytotoxicity of rim domain loop-exchange mutants between LukE and Hlg2 indicated that loop-4 of LukE is essential for cytotoxicity in combination with LukD. Interestingly, Hlg2 showed LukF-dependent CCR5-mediated cytotoxicity, suggesting that the F-components of toxins also play a role in the cell-specific cytotoxicity.

KW - CCR5

KW - Cytotoxicity

KW - Staphylococcal β-PFTs

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JO - Bioscience, Biotechnology and Biochemistry

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ER -

Cytotoxicity analysis of staphylococcal bi-component β-pore forming toxins using the CHO cells expressing human lymphocyte receptor CCR5

Abstract

Keywords

ASJC Scopus subject areas

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