Damage to red blood cells induced by acoustic cavitation

S. Daniels; T. Kodama; D. J. Price

doi:10.1016/0301-5629(94)00089-1

Damage to red blood cells induced by acoustic cavitation

S. Daniels, T. Kodama, D. J. Price

MEN - Biomedical Engineering

Cardiff University

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

This experimental study has revealed damage to red blood cells that is quantitatively related to the acoustic pressure during irradiation with 0.75-MHz continuous-wave ultrasound, using a range of intensities comparable to those employed by ultrasonic physiotherapy equipment (0.25 to 7 W cm^-2 spatial average temporal average). Damage to the red blood cells was investigated by measuring the extent of haemolysis using a UV/VIS spectrophotometer. There was a clear correlation between the amount of haemoglobin released and the intensity of harmonic emissions recorded from the sample during irradiation. The observed degree of haemolysis could not be produced by temperature rises in the absence of the sound field. This suggests that the damage was a direct result of acoustic cavitation.

Original language	English
Pages (from-to)	105-111
Number of pages	7
Journal	Ultrasound in Medicine and Biology
Volume	21
Issue number	1
DOIs	https://doi.org/10.1016/0301-5629(94)00089-1
Publication status	Published - 1995

Keywords

Absorption spectroscopy
Bubbles
Cavitation
Haemoglobin
Haemolysis
Red blood cells
Ultrasound

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10.1016/0301-5629(94)00089-1

Cite this

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title = "Damage to red blood cells induced by acoustic cavitation",

abstract = "This experimental study has revealed damage to red blood cells that is quantitatively related to the acoustic pressure during irradiation with 0.75-MHz continuous-wave ultrasound, using a range of intensities comparable to those employed by ultrasonic physiotherapy equipment (0.25 to 7 W cm-2 spatial average temporal average). Damage to the red blood cells was investigated by measuring the extent of haemolysis using a UV/VIS spectrophotometer. There was a clear correlation between the amount of haemoglobin released and the intensity of harmonic emissions recorded from the sample during irradiation. The observed degree of haemolysis could not be produced by temperature rises in the absence of the sound field. This suggests that the damage was a direct result of acoustic cavitation.",

keywords = "Absorption spectroscopy, Bubbles, Cavitation, Haemoglobin, Haemolysis, Red blood cells, Ultrasound",

author = "S. Daniels and T. Kodama and Price, {D. J.}",

note = "Funding Information: Acknowledgements-The experimental work described in this article was completed when the Hyperbaric Group was located in the Physical Chemistry Laboratory, University of Oxford. Financial support for this work was provided by the Wellcome Trust. T. Kodama was a visiting scientific fellow in the Hyperbaric Group.",

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AU - Kodama, T.

AU - Price, D. J.

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PY - 1995

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N2 - This experimental study has revealed damage to red blood cells that is quantitatively related to the acoustic pressure during irradiation with 0.75-MHz continuous-wave ultrasound, using a range of intensities comparable to those employed by ultrasonic physiotherapy equipment (0.25 to 7 W cm-2 spatial average temporal average). Damage to the red blood cells was investigated by measuring the extent of haemolysis using a UV/VIS spectrophotometer. There was a clear correlation between the amount of haemoglobin released and the intensity of harmonic emissions recorded from the sample during irradiation. The observed degree of haemolysis could not be produced by temperature rises in the absence of the sound field. This suggests that the damage was a direct result of acoustic cavitation.

AB - This experimental study has revealed damage to red blood cells that is quantitatively related to the acoustic pressure during irradiation with 0.75-MHz continuous-wave ultrasound, using a range of intensities comparable to those employed by ultrasonic physiotherapy equipment (0.25 to 7 W cm-2 spatial average temporal average). Damage to the red blood cells was investigated by measuring the extent of haemolysis using a UV/VIS spectrophotometer. There was a clear correlation between the amount of haemoglobin released and the intensity of harmonic emissions recorded from the sample during irradiation. The observed degree of haemolysis could not be produced by temperature rises in the absence of the sound field. This suggests that the damage was a direct result of acoustic cavitation.

KW - Absorption spectroscopy

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KW - Cavitation

KW - Haemoglobin

KW - Haemolysis

KW - Red blood cells

KW - Ultrasound

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Damage to red blood cells induced by acoustic cavitation

Abstract

Keywords

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