TY - JOUR
T1 - Data processing pipeline for serial femtosecond crystallography at SACLA
AU - Nakane, Takanori
AU - Joti, Yasumasa
AU - Tono, Kensuke
AU - Yabashi, Makina
AU - Nango, Eriko
AU - Iwata, So
AU - Ishitani, Ryuichiro
AU - Nureki, Osamu
N1 - Publisher Copyright:
© 2016 International Union of Crystallography.
PY - 2016
Y1 - 2016
N2 - A data processing pipeline for serial femtosecond crystallography at SACLA was developed, based on Cheetah [Barty et al. (2014). J. Appl. Cryst. 47, 1118-1131] and CrystFEL [White et al. (2016). J. Appl. Cryst. 49, 680-689]. The original programs were adapted for data acquisition through the SACLA API, thread and inter-node parallelization, and efficient image handling. The pipeline consists of two stages: The first, online stage can analyse all images in real time, with a latency of less than a few seconds, to provide feedback on hit rate and detector saturation. The second, offline stage converts hit images into HDF5 files and runs CrystFEL for indexing and integration. The size of the filtered compressed output is comparable to that of a synchrotron data set. The pipeline enables real-time feedback and rapid structure solution during beamtime.
AB - A data processing pipeline for serial femtosecond crystallography at SACLA was developed, based on Cheetah [Barty et al. (2014). J. Appl. Cryst. 47, 1118-1131] and CrystFEL [White et al. (2016). J. Appl. Cryst. 49, 680-689]. The original programs were adapted for data acquisition through the SACLA API, thread and inter-node parallelization, and efficient image handling. The pipeline consists of two stages: The first, online stage can analyse all images in real time, with a latency of less than a few seconds, to provide feedback on hit rate and detector saturation. The second, offline stage converts hit images into HDF5 files and runs CrystFEL for indexing and integration. The size of the filtered compressed output is comparable to that of a synchrotron data set. The pipeline enables real-time feedback and rapid structure solution during beamtime.
KW - Parallelization
KW - Real-time processing
KW - SACLA
KW - Serial femtosecond crystallography
KW - Spot finding
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U2 - 10.1107/S1600576716005720
DO - 10.1107/S1600576716005720
M3 - Article
AN - SCOPUS:84973304283
SN - 0021-8898
VL - 49
SP - 1035
EP - 1041
JO - Journal of Applied Crystallography
JF - Journal of Applied Crystallography
ER -