Defective mononuclear cell antibody-dependent cellular cytotoxicity (adcc) in patients with leukocyte adhesion deficiency emphasizing on different cd11/cd18 requirement of fcγri versus fcγrii in adcc

Toshiro Majima; Yoshiyuki Ohashi; Ryoichi Nagatomi; Atsuo Iizuka; Tasuke Konno

doi:10.1006/cimm.1993.1120

Defective mononuclear cell antibody-dependent cellular cytotoxicity (adcc) in patients with leukocyte adhesion deficiency emphasizing on different cd11/cd18 requirement of fcγri versus fcγrii in adcc

Toshiro Majima, Yoshiyuki Ohashi, Ryoichi Nagatomi, Atsuo Iizuka, Tasuke Konno

MEN - Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The defective antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells (MNC) from patients with leukocyte adhesion deficiency (LAD), β₂integrins (CD11a-c/CD18) deficiency was shown. LAD patients completely failed to generate MNC-ADCC against sheep red blood cells (SRBC) sensitized with murine (m) IgG2b, but had diminished but significant cytolysis against mIgG2a-SRBC, suggesting that the CD11/CD18 requirement of FcγRI is different from that of FcγRII in MNC-ADCC. Blocking experiments with monoclonal antibodies (mAb) against individual subunits of CD11/CD18 revealed that anti-CD18 mAb almost completely inhibited mlgG2b-mediated ADCC by normal monocytes, but only partially inhibited mlgG2a-mediated ADCC. These data may confirm the evidence that FcγRII-mediated ADCC absolutely requires CD11/CD18 but FcγRI-mediated ADCC does not. Among subunits of CD11/CD18, CD18 appeared to be most involved in lysis of sensitized SRBC.

Original language	English
Pages (from-to)	385-396
Number of pages	12
Journal	Cellular Immunology
Volume	148
Issue number	2
DOIs	https://doi.org/10.1006/cimm.1993.1120
Publication status	Published - 1993 May

ASJC Scopus subject areas

Immunology

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Defective mononuclear cell antibody-dependent cellular cytotoxicity (adcc) in patients with leukocyte adhesion deficiency emphasizing on different cd11/cd18 requirement of fcγri versus fcγrii in adcc. / Majima, Toshiro; Ohashi, Yoshiyuki; Nagatomi, Ryoichi et al.
In: Cellular Immunology, Vol. 148, No. 2, 05.1993, p. 385-396.

Research output: Contribution to journal › Article › peer-review

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abstract = "The defective antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells (MNC) from patients with leukocyte adhesion deficiency (LAD), β2integrins (CD11a-c/CD18) deficiency was shown. LAD patients completely failed to generate MNC-ADCC against sheep red blood cells (SRBC) sensitized with murine (m) IgG2b, but had diminished but significant cytolysis against mIgG2a-SRBC, suggesting that the CD11/CD18 requirement of FcγRI is different from that of FcγRII in MNC-ADCC. Blocking experiments with monoclonal antibodies (mAb) against individual subunits of CD11/CD18 revealed that anti-CD18 mAb almost completely inhibited mlgG2b-mediated ADCC by normal monocytes, but only partially inhibited mlgG2a-mediated ADCC. These data may confirm the evidence that FcγRII-mediated ADCC absolutely requires CD11/CD18 but FcγRI-mediated ADCC does not. Among subunits of CD11/CD18, CD18 appeared to be most involved in lysis of sensitized SRBC.",

author = "Toshiro Majima and Yoshiyuki Ohashi and Ryoichi Nagatomi and Atsuo Iizuka and Tasuke Konno",

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AB - The defective antibody-dependent cellular cytotoxicity (ADCC) of mononuclear cells (MNC) from patients with leukocyte adhesion deficiency (LAD), β2integrins (CD11a-c/CD18) deficiency was shown. LAD patients completely failed to generate MNC-ADCC against sheep red blood cells (SRBC) sensitized with murine (m) IgG2b, but had diminished but significant cytolysis against mIgG2a-SRBC, suggesting that the CD11/CD18 requirement of FcγRI is different from that of FcγRII in MNC-ADCC. Blocking experiments with monoclonal antibodies (mAb) against individual subunits of CD11/CD18 revealed that anti-CD18 mAb almost completely inhibited mlgG2b-mediated ADCC by normal monocytes, but only partially inhibited mlgG2a-mediated ADCC. These data may confirm the evidence that FcγRII-mediated ADCC absolutely requires CD11/CD18 but FcγRI-mediated ADCC does not. Among subunits of CD11/CD18, CD18 appeared to be most involved in lysis of sensitized SRBC.

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Defective mononuclear cell antibody-dependent cellular cytotoxicity (adcc) in patients with leukocyte adhesion deficiency emphasizing on different cd11/cd18 requirement of fcγri versus fcγrii in adcc

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