Degassing-assisted patterning of cell culture surfaces

Chunxiong Luo, Xiaofang Ni, Li Liu, Shinichiro Nomura, Yong Chen

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


We developed an alternative patterning technique which is capable of producing both topographic and biochemical features for cell culture studies. This technique is based on microaspiration induced with a degassed poly (dimethylsiloxane) (PDMS) mold. After degassing in a rough vacuum chamber and placed on a sample surface, liquid solution can be aspired through channels and cavities created in the PDMS mold. Depending on the composition of the solution and the associated drying or incubation processes, a variety of surface patterns can be produced without applying external pressure. For demonstration, we fabricated agarose gel microwells and biomolecule patterns either on a glass plate or in a cell culture Petri dish, both applicable for cell culture studies.

Original languageEnglish
Pages (from-to)854-859
Number of pages6
JournalBiotechnology and Bioengineering
Issue number4
Publication statusPublished - 2010 Mar 1
Externally publishedYes


  • Cell culture
  • Soft lithography
  • Surface patterning

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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