Deletion mapping of 14q32 in human neuroblastoma defines an 1,100-kb region of common allelic loss

Masato Hoshi; Hiromi O. Shiwaku; Yutaka Hayashi; Yasuhiko Kaneko; Akira Horii

doi:10.1002/1096-911X(20001201)35:6<522::AID-MPO4>3.0.CO;2-6

Deletion mapping of 14q32 in human neuroblastoma defines an 1,100-kb region of common allelic loss

Masato Hoshi, Hiromi O. Shiwaku, Yutaka Hayashi, Yasuhiko Kaneko, Akira Horii

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Background. We analyzed loss of heterozygosity (LOH) in 54 primary neuroblastomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) NBs showed LOH. Procedure. The smallest region of overlap (SRO) was identified between D14S62 and D14S987. Results. There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN, and ploidy. A sequence-ready bacterial artificial chromosome (BAC) contig was constructed, and the minimum tiling path of six BACs covered the SRO; the physical length of this region was no larger than 1,000 kb. Conclusions. Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB. (C) 2000 Wiley-Liss, Inc.

Original language	English
Pages (from-to)	522-525
Number of pages	4
Journal	Medical and Pediatric Oncology
Volume	35
Issue number	6
DOIs	https://doi.org/10.1002/1096-911X(20001201)35:6<522::AID-MPO4>3.0.CO;2-6
Publication status	Published - 2000

Keywords

BAC
Chromosome arm 14q
LOH
Neuroblastoma
Tumor suppressor gene

ASJC Scopus subject areas

Pediatrics, Perinatology, and Child Health
Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/1096-911X(20001201)35:6<522::AID-MPO4>3.0.CO;2-6

Cite this

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title = "Deletion mapping of 14q32 in human neuroblastoma defines an 1,100-kb region of common allelic loss",

abstract = "Background. We analyzed loss of heterozygosity (LOH) in 54 primary neuroblastomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) NBs showed LOH. Procedure. The smallest region of overlap (SRO) was identified between D14S62 and D14S987. Results. There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN, and ploidy. A sequence-ready bacterial artificial chromosome (BAC) contig was constructed, and the minimum tiling path of six BACs covered the SRO; the physical length of this region was no larger than 1,000 kb. Conclusions. Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB. (C) 2000 Wiley-Liss, Inc.",

keywords = "BAC, Chromosome arm 14q, LOH, Neuroblastoma, Tumor suppressor gene",

author = "Masato Hoshi and Shiwaku, {Hiromi O.} and Yutaka Hayashi and Yasuhiko Kaneko and Akira Horii",

year = "2000",

doi = "10.1002/1096-911X(20001201)35:6<522::AID-MPO4>3.0.CO;2-6",

language = "English",

volume = "35",

pages = "522--525",

journal = "Medical and Pediatric Oncology",

issn = "1545-5009",

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TY - JOUR

T1 - Deletion mapping of 14q32 in human neuroblastoma defines an 1,100-kb region of common allelic loss

AU - Hoshi, Masato

AU - Shiwaku, Hiromi O.

AU - Hayashi, Yutaka

AU - Kaneko, Yasuhiko

AU - Horii, Akira

PY - 2000

Y1 - 2000

N2 - Background. We analyzed loss of heterozygosity (LOH) in 54 primary neuroblastomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) NBs showed LOH. Procedure. The smallest region of overlap (SRO) was identified between D14S62 and D14S987. Results. There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN, and ploidy. A sequence-ready bacterial artificial chromosome (BAC) contig was constructed, and the minimum tiling path of six BACs covered the SRO; the physical length of this region was no larger than 1,000 kb. Conclusions. Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB. (C) 2000 Wiley-Liss, Inc.

AB - Background. We analyzed loss of heterozygosity (LOH) in 54 primary neuroblastomas (NBs) using 12 microsatellite markers on 14q32, and found that 31% (17/54) NBs showed LOH. Procedure. The smallest region of overlap (SRO) was identified between D14S62 and D14S987. Results. There was no statistical correlation between LOH and any clinicopathologic features, including age, stage, amplification of MYCN, and ploidy. A sequence-ready bacterial artificial chromosome (BAC) contig was constructed, and the minimum tiling path of six BACs covered the SRO; the physical length of this region was no larger than 1,000 kb. Conclusions. Our findings support the existence of a putative tumor-related gene on 14q32 for the tumorigenesis of NB. (C) 2000 Wiley-Liss, Inc.

KW - BAC

KW - Chromosome arm 14q

KW - LOH

KW - Neuroblastoma

KW - Tumor suppressor gene

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DO - 10.1002/1096-911X(20001201)35:6<522::AID-MPO4>3.0.CO;2-6

M3 - Article

C2 - 11107107

AN - SCOPUS:0033653344

SN - 1545-5009

VL - 35

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JO - Medical and Pediatric Oncology

JF - Medical and Pediatric Oncology

IS - 6

ER -

Deletion mapping of 14q32 in human neuroblastoma defines an 1,100-kb region of common allelic loss

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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